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- Title
- Improved survival and developmental rates in vitrified-warmed pig oocytes after recovery culture with coenzyme Q10
- Author(s)
- I S Hwang; D J Kwon; T W Kwak; Jeong Woong Lee; G S Im; S Hwang
- Bibliographic Citation
- Cryoletters, vol. 37, no. 1, pp. 59-67
- Publication Year
- 2016
- Abstract
- BACKGROUND:
The primary problems with porcine oocyte vitrification are their low viability and development; both need improvement.
OBJECTIVE:
This study was designed to improve the survival and developmental rates in vitrified-warmed porcine oocytes.
MATERIALS AND METHODS:
Porcine oocytes matured in vitro were vitrified-warmed with Cryotop. Then the oocytes were supplemented with Q10 during recovery culture.
RESULTS:
The survival rates immediately after warming were 92.9% by morphological inspection and 39.3% by fluorescein diacetate (FDA) assay. The group of recovery culture with Q10 (VC+Q10) showed significantly higher viability compared to the group of recovery culture without Q10 (VC+) analyzed by morphology and the FDA. The VC+Q10 group showed a low Bax/Bcl-xl ratio and a high expression of MAP3K12 and TGFB3 compared to the VC+. The cleavage rate did not differ in both groups but, blastocyst yield was higher in VC+Q10 than the VC+ group.
CONCLUSION:
Supplementation of Q10 during recovery culture led to a higher blastocyst yield by increasing survival rates and regulating mRNA expressions.
- Keyword
- AntioxidantCoenzyme Q10CryopreservationPig oocyteVitrification
- ISSN
- 0143-2044
- Publisher
- Cryo Letters
- DOI
- http://dx.doi.org/10.12750/JET.2016.31.2.117
- Type
- Article
- Appears in Collections:
- Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
- Files in This Item:
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