Improved survival and developmental rates in vitrified-warmed pig oocytes after recovery culture with coenzyme Q10

Cited 0 time in scopus
Metadata Downloads
Title
Improved survival and developmental rates in vitrified-warmed pig oocytes after recovery culture with coenzyme Q10
Author(s)
I S Hwang; D J Kwon; T W Kwak; Jeong Woong Lee; G S Im; S Hwang
Bibliographic Citation
Cryoletters, vol. 37, no. 1, pp. 59-67
Publication Year
2016
Abstract
BACKGROUND: The primary problems with porcine oocyte vitrification are their low viability and development; both need improvement. OBJECTIVE: This study was designed to improve the survival and developmental rates in vitrified-warmed porcine oocytes. MATERIALS AND METHODS: Porcine oocytes matured in vitro were vitrified-warmed with Cryotop. Then the oocytes were supplemented with Q10 during recovery culture. RESULTS: The survival rates immediately after warming were 92.9% by morphological inspection and 39.3% by fluorescein diacetate (FDA) assay. The group of recovery culture with Q10 (VC+Q10) showed significantly higher viability compared to the group of recovery culture without Q10 (VC+) analyzed by morphology and the FDA. The VC+Q10 group showed a low Bax/Bcl-xl ratio and a high expression of MAP3K12 and TGFB3 compared to the VC+. The cleavage rate did not differ in both groups but, blastocyst yield was higher in VC+Q10 than the VC+ group. CONCLUSION: Supplementation of Q10 during recovery culture led to a higher blastocyst yield by increasing survival rates and regulating mRNA expressions.
Keyword
AntioxidantCoenzyme Q10CryopreservationPig oocyteVitrification
ISSN
0143-2044
Publisher
Cryo Letters
DOI
http://dx.doi.org/10.12750/JET.2016.31.2.117
Type
Article
Appears in Collections:
Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.