Therapeutic and prophylactic activity of itraconazole against human rhinovirus infection in a murine model = 이트라코나졸의 감기바이러스 억제효능

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Title
Therapeutic and prophylactic activity of itraconazole against human rhinovirus infection in a murine model = 이트라코나졸의 감기바이러스 억제효능
Author(s)
A Shim; J H Song; B E Kwon; J J Lee; J H Ahn; Y J Kim; K J Rhee; S Y Chang; Y Cha; Y S Lee; M N Kwon; K S Park; Dong-Eun Kim; Sungchan Cho; H J Cho; H J Ko
Bibliographic Citation
Scientific Reports, vol. 6, pp. 23110-23110
Publication Year
2016
Abstract
Human rhinovirus (HRV) is the most common viral infectious agent in humans and is the predominant cause of the common cold. There is a need for appropriate vaccines or therapeutic agents to treat HRV infection. In this study, we investigated whether itraconazole (ICZ) can protect cells from HRV-induced cytotoxicity. Replication of HRV1B was reduced by ICZ treatment in the lungs of HRV1B- as compared to vehicle-treated mice. The numbers of immune cells, including granulocytes and monocytes, were reduced in bronchoalveolar lavage fluid (BALF) by ICZ administration after HRV1B infection, corresponding to decreased pro-inflammatory cytokine and chemokine levels in BALF. A histological analysis of lung tissue showed that ICZ suppressed inflammation caused by HRV1B infection. Interestingly, pretreatment of mice with ICZ in the form of a nasal spray had potent prophylactic antiviral activity. Cholesterol accumulation in the plasma membrane was observed upon HRV infection; ICZ blocked cholesterol trafficking to the plasma membrane, as well as resulted in its accumulation in subcellular compartments near the nucleus. These findings suggest that ICZ is a potential antiviral agent for the treatment of HRV infection, which can be adopted preventatively as well as therapeutically.
ISSN
2045-2322
Publisher
Springer-Nature Pub Group
DOI
http://dx.doi.org/10.1038/srep23110
Type
Article
Appears in Collections:
Ochang Branch Institute > Natural Medicine Research Center > 1. Journal Articles
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