Cytokine-induced killer cells interact with tumor lysate?pulsed dendritic cells via CCR5 signaling

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Title
Cytokine-induced killer cells interact with tumor lysate?pulsed dendritic cells via CCR5 signaling
Author(s)
H K Lee; Y G Kim; J S Kim; E J Park; B Kim; K H Park; Jong Soon Kang; J T Hong; Y Kim; S B Han
Bibliographic Citation
Cancer Letters, vol. 378, no. 2, pp. 142-149
Publication Year
2016
Abstract
The antitumor activity of cytokine-induced killer (CIK) cells can be increased by co-culturing them with tumor lysate?pulsed dendritic cells (tDCs); this phenomenon has been studied mainly at the population level. Using time-lapse imaging, we examined how CIK cells gather information from tDCs at the single-cell level. tDCs highly expressed CCL5, which bound CCR5 expressed on CIK cells. tDCs strongly induced migration of Ccr5+/+ CIK cells, but not that of Ccr5?/? CIK cells or Ccr5+/+ CIK cells treated with the CCR5 antagonist Maraviroc. Individual tDCs contacted Ccr5+/+ CIK cells more frequently and lengthily than with Ccr5?/? CIK cells. Consequently, tDCs increased the antitumor activity of Ccr5+/+ CIK cells in vitro and in vivo, but did not increase that of Ccr5?/? CIK cells. Taken together, our data provide insight into the mechanism of CIK cell activation by tDCs at the single-cell level.
Keyword
Cellcell interactionMigrationTime-lapse imaging
ISSN
0304-3835
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.canlet.2016.05.020
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Laboratory Animal Resource Center > 1. Journal Articles
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