Direct protein?protein conjugation by genetically introducing bioorthogonal functional groups into proteins

Cited 7 time in scopus
Metadata Downloads
Title
Direct protein?protein conjugation by genetically introducing bioorthogonal functional groups into proteins
Author(s)
S Kim; W Ko; Bong Hyun Sung; S C Kim; H S Lee
Bibliographic Citation
Bioorganic & Medicinal Chemistry, vol. 24, no. 22, pp. 5816-5822
Publication Year
2016
Abstract
Proteins often function as complex structures in conjunction with other proteins. Because these complex structures are essential for sophisticated functions, developing protein?protein conjugates has gained research interest. In this study, site-specific protein?protein conjugation was performed by genetically incorporating an azide-containing amino acid into one protein and a bicyclononyne (BCN)-containing amino acid into the other. Three to four sites in each of the proteins were tested for conjugation efficiency, and three combinations showed excellent conjugation efficiency. The genetic incorporation of unnatural amino acids (UAAs) is technically simple and produces the mutant protein in high yield. In addition, the conjugation reaction can be conducted by simple mixing, and does not require additional reagents or linker molecules. Therefore, this method may prove very useful for generating protein?protein conjugates and protein complexes of biochemical significance
Keyword
Click reactionGenetic incorporationProteinprotein conjugationUnnatural amino acids
ISSN
0968-0896
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.bmc.2016.09.035
Type
Article
Appears in Collections:
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.