Structural features of influenza A virus panhandle RNA enabling the activation of RIG-I independently of 5'-triphosphate = 5' 말단 삼인산염과 관계없이 RIG-I를 활성화시키는 인플루엔자 A 바이러스의 팬핸들 RNA의 구조적 특성

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Title
Structural features of influenza A virus panhandle RNA enabling the activation of RIG-I independently of 5'-triphosphate = 5' 말단 삼인산염과 관계없이 RIG-I를 활성화시키는 인플루엔자 A 바이러스의 팬핸들 RNA의 구조적 특성
Author(s)
Mi-Kyung Lee; H E Kim; E B Park; J Lee; K H Kim; K Lim; S Yum; Y H Lee; S J Kang; J H Lee; B S Choi
Bibliographic Citation
Nucleic Acids Research, vol. 44, no. 17, pp. 8407-8416
Publication Year
2016
Abstract
Retinoic acid-inducible gene I (RIG-I) recognizes specific molecular patterns of viral RNAs for inducing type I interferon. The C-terminal domain (CTD) of RIG-I binds to double-stranded RNA (dsRNA) with the 5'-triphosphate (5'-PPP), which induces a conformational change in RIG-I to an active form. It has been suggested that RIG-I detects infection of influenza A virus by recognizing the 5'-triphosphorylated panhandle structure of the viral RNA genome. Influenza panhandle RNA has a unique structure with a sharp helical bending. In spite of extensive studies of how viral RNAs activate RIG-I, whether the structural elements of the influenza panhandle RNA confer the ability to activate RIG-I signaling has been poorly explored. Here, we investigated the dynamics of the influenza panhandle RNA in complex with RIG-I CTD using NMR spectroscopy and showed that the bending structure of the panhandle RNA negates the requirement of a 5'-PPP moiety for RIG-I activation.
ISSN
0305-1048
Publisher
Oxford Univ Press
DOI
http://dx.doi.org/10.1093/nar/gkw525
Type
Article
Appears in Collections:
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
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