Increased expression of herpes virus-encoded hsv1-miR-H18 and hsv2-miR-H9-5p in cancer-containing prostate tissue compared to that in benign prostate hyperplasia tissue

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Title
Increased expression of herpes virus-encoded hsv1-miR-H18 and hsv2-miR-H9-5p in cancer-containing prostate tissue compared to that in benign prostate hyperplasia tissue
Author(s)
S J Yun; P Jeong; H W Kang; H K Shinn; Y H Kim; C Yan; Y K Choi; D Kim; D H Ryu; Y S Ha; T H Kim; T G Kwon; J M Kim; S H Suh; Seon-Kyu KimSeon-Young Kim; S T Kim; W T Kim; O J Lee; S K Moon; N H Kim; I Y Kim; J Kim; H J Cha; Y H Choi; E J Cha; W J Kim
Bibliographic Citation
International Neurourology Journal, vol. 20, pp. 122-130
Publication Year
2016
Abstract
Purpose: Previously, we reported the presence of virus-encoded microRNAs (miRNAs) in the urine of prostate cancer (CaP) patients. In this study, we investigated the expression of two herpes virus-encoded miRNAs in prostate tissue. Methods: A total of 175 tissue samples from noncancerous benign prostatic hyperplasia (BPH), 248 tissue samples from patients with CaP and BPH, and 50 samples from noncancerous surrounding tissues from these same patients were analyzed for the expression of two herpes virus-encoded miRNAs by real-time polymerase chain reaction (PCR) and immunocytochemistry using nanoparticles as molecular beacons. Results: Real-time reverse transcription-PCR results revealed significantly higher expression of hsv1-miR-H18 and hsv2-miRH9- 5p in surrounding noncancerous and CaP tissues than that in BPH tissue (each comparison, P < 0.001). Of note, these miRNA were expressed equivalently in the CaP tissues and surrounding noncancerous tissues. Moreover, immunocytochemistry clearly demonstrated a significant enrichment of both hsv1-miR-H18 and hsv2-miR-H9 beacon-labeled cells in CaP and surrounding noncancerous tissue compared to that in BPH tissue (each comparison, P < 0.05 for hsv1-miR-H18 and hsv2- miR-H9). Conclusions: These results suggest that increased expression of hsv1-miR-H18 and hsv2-miR-H95p might be associated with tumorigenesis in the prostate. Further studies will be required to elucidate the role of these miRNAs with respect to CaP and herpes viral infections.
Keyword
HerpesviridaeMicroRNAsProstate HyperplasiaProstate Neoplasms
ISSN
2093-4777
Publisher
South Korea
DOI
http://dx.doi.org/10.5213/inj.1632552.276
Type
Article
Appears in Collections:
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
Korea Bioinformation Center > 1. Journal Articles
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