Humulus japonicus inhibits the progression of Alzheimer's disease in a APP/PS1 transgenic mouse model

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Title
Humulus japonicus inhibits the progression of Alzheimer's disease in a APP/PS1 transgenic mouse model
Author(s)
Tae-Shin Park; Young-Kyoung Ryu; Hye-Yeon Park; J Y Kim; Jun Go; Jung Ran NohYong-Hoon KimJung Hwan HwangDong Hee Choi; W K Oh; Chul Ho LeeKyoung Shim Kim
Bibliographic Citation
International Journal of Molecular Medicine, vol. 39, no. 1, pp. 21-30
Publication Year
2017
Abstract
Humulus japonicus Siebold and Zucc. (HJ) has traditionally been administered to patients with pulmonary disease, skin disease and hypertension in Korea, and it is considered to exert anti-inflammatory, antioxidant, antimicrobial and antimycobacterial effects. However, its effects against Alzheimer's disease (AD) have yet to be explored. Thus, this study was carried out to investigate whether HJ has a beneficial effect on the progression of AD in an animal model. A methanolic extract of HJ (500 mg/kg/day) was intragastrically administered to 5-month-old APP/PS1 transgenic (Tg-APP/PS1) mice for 2.5 months. Novel object recognition and Y-maze alteration tests were used to assess cognitive function, and an immunohistochemical assay was performed to assess amyloid β (Aβ)deposition, tau phosphorylation and gliosis. An in vitro assay using a microglial cell line was also performed to investigate the anti.inflammatory effects of HJ. Our results revealed that HJ significantly decreased the mRNA and protein expression levels of tumor necrosis factor-α (TNF.α), interleukin (IL)-1β, IL-6 and inducible nitric oxide synthase (iNOS) induced by lipopolysaccharide in the microglial cell line. The administration of HJ for 2 months improved the cognitive function of Tg-APP/PS1 mice. HJ notably reduced the area occupied by Aβ and neurofibrillary tangles, and the number of activated astrocytes and microglia in the cortex of Tg-APP/PS1 mice. The findings of our study suggest that HJ has the therapeutic potential to inhibit the progression of AD and to improve cognitive deterioration in Tg-APP/PS1 mice.
Keyword
Alzheimer's diseaseCognitionHumulus japonicusInflammationα-amyloid plaque
ISSN
1107-3756
Publisher
Spandidos Publ Ltd
DOI
http://dx.doi.org/10.3892/ijmm.2016.2804
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Laboratory Animal Resource Center > 1. Journal Articles
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