One-step purification of melittin derived from Apis mellifera bee venom

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Title
One-step purification of melittin derived from Apis mellifera bee venom
Author(s)
A C L Teoh; Kyoung Hwa RyuEun Gyo Lee
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 27, no. 1, pp. 84-91
Publication Year
2017
Abstract
The concern over the use of melittin in honey bee venom due to its adverse reaction caused by allergens such as phospholipase A2 (PLA) and hyaluronidase (HYA) has been an obstacle towards its usage. We developed a novel single-step method for melittin purification and the2 removal of PLA and HYA. This study explores the influence of pH, buffer compositions, salt concentration, and types of cation-exchange chromatography resins on the recovery of2 melittin and the removal of both HYA and PLA. Melittin was readily purified with a strong cation-exchange resin at pH 6.0 with sodium phosphate buffer. It resulted in a recovery yield2 of melittin up to 93% (5.87 mg from a total of 6.32 mg of initial melittin in crude bee venom), which is higher than any previously reported studies on melittin purification. PLA (99%) and HYA (96%) were also successfully removed. Our study generates a single-step purification2 method for melittin with a high removal rate of PLA and HYA, enabling melittin to be fully utilized for its therapeutic purposes.
Keyword
Cation-exchange chromatographyHyaluronidaseMelittinPhospholipase A2Purification
ISSN
1017-7825
Publisher
South Korea
DOI
http://dx.doi.org/10.4014/jmb.1608.08042
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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