Establishment and characterization of immortalized minipig neural stem cell line = 불멸화 미니돼지 신경줄기세포주의 확립 및 특성 분석

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Title
Establishment and characterization of immortalized minipig neural stem cell line = 불멸화 미니돼지 신경줄기세포주의 확립 및 특성 분석
Author(s)
S S Choi; Seung-Bin YoonSang-Rae LeeSun-Uk Kim; Y J Cha; D Lee; S U Kim; Kyu Tae Chang; H J Lee
Bibliographic Citation
Cell Transplantation, vol. 26, pp. 271-281
Publication Year
2017
Abstract
Despite the increasing importance of minipigs in biomedical research, there has been relatively little research concerning minipig-derived adult stem cells as a promising research tool that could be used to develop stem cell-based therapies. We first generated immortalized neural stem cells (iNSCs) from primary minipig olfactory bulb cells (pmpOBCs) and defined the characteristics of the cell line. Primary neural cells were prepared from minipig neonate olfactory bulbs and immortalized by infection with retrovirus carrying the v-myc gene. The minipig iNSCs (mpiNSCs) had normal karyotypes and expressed NSC-specific markers, including nestin, vimentin, Musashi1, and SOX2, suggesting a similarity to human NSCs. On the basis of the global gene expression profiles from the microarray analysis, neurogenesis-associated transcript levels were predominantly altered in mpiNSCs compared with pmpOBCs. These findings increase our understanding of minipig stem cells and contribute to the utility of mpiNSCs as resources for immortalized stem cell experiments.
Keyword
ImmortalizationMinipigNeural stem cells (NSCs)Olfactory bulbv-myc
ISSN
0963-6897
Publisher
Sage
DOI
http://dx.doi.org/10.3727/096368916X692852
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Primate Resources Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > National Primate Research Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
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