Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR = 실시간 유전자 증폭법을 활용한 사스, 메르스 및 관련 박쥐 코로나바이러스 동시검출 기법

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Title
Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR = 실시간 유전자 증폭법을 활용한 사스, 메르스 및 관련 박쥐 코로나바이러스 동시검출 기법
Author(s)
Ji Yeong Noh; Sun-Woo YoonDoo-Jin KimMoo-Seung Lee; Ji-Hyung Kim; W Na; D Song; Dae Gwin Jeong; Hye Kwon Kim
Bibliographic Citation
Archives of Virology, vol. 162, no. 6, pp. 1617-1623
Publication Year
2017
Abstract
Since severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS) coronaviruses (CoVs) share similar characteristics with respect to clinical signs, etiology, and transmission, methods for a rapid and accurate differential diagnosis are important. Therefore, the aim of this study was to develop a duplex real-time reverse transcription (RT)-PCR method for the simultaneous detection of these viruses. Primers and probes that target the conserved spike S2 region of human SARS-CoV, MERS-CoV, and their related bat CoVs were designed. The results of real-time RT-PCR showed specific reactions for each virus with adequate detection limits of 50-100 copies/mL and 5-100 copies/mL using pUC57-SARS-pS2 (a template for SARS-CoV) and pGEM-MERS-S2 (a template for MERS-CoV), respectively. In addition, this real-time RT-PCR system was able to detect the target viruses SARS-like bat CoV and MERS-CoV in bat fecal samples and sputum of MERS patients, respectively. Therefore, this newly developed real-time RT-PCR method is expected to detect not only SARS-CoV and MERS-CoV in humans but also several bat CoVs that are closely related to these viruses in bats. ⓒ 2017, Springer-Verlag Wien.
ISSN
0304-8608
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s00705-017-3281-9
Type
Article
Appears in Collections:
Division of Biomaterials Research > Bionanotechnology Research Center > 1. Journal Articles
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
Division of Research on National Challenges > Environmental diseases research center > 1. Journal Articles
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