A surface plasmon resonance (SPR) sensor for detection of toluene (C6H5CH3)

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A surface plasmon resonance (SPR) sensor for detection of toluene (C6H5CH3)
T T Dung; Moonil Kim
Bibliographic Citation
Applied Science and Convergence Technology, vol. 27, no. 6, pp. 184-188
Publication Year
Herein, we report a novel surface plasmon resonance (SPR) sensor modified with toluene binding domain (TBD) to detect toluene. The toluene binding domain was manipulated with cysteines for rapid, easy and orientation-controlled immobilization of the protein onto a SPR gold surface due to the specific interaction between a bare gold and the thiol group (-SH) of cysteines. After the purification and on-chip immobilization of the recombinant TBD, the thickness of cysteine-tagged TBD was determined to be approximately 1.8 nm according to the cross-sectional analysis by atomic force microscopy (AFM). The SPR analysis of cysteine-tagged TBD adsorption showed that cysteine-tagged TBD bound onto a gold thin film with high binding affinity. With a SPR-based ligand binding assay, a detectable increase in the SPR response was observed with 15.62 uM of toluene. Due to the inherent specificity of TBD, this method showed a comparative specificity for the determination of toluene, as it exhibited no SPR response to aromatic compounds such as p-xylene and benzene. Taken together, the results obtained from this study indicate that TBD-modified SPR sensor system can be useful for detecting toluene, a volatile organic compound widely distributed in the environment.
BiosensorSurface plasmon resonanceSPRTolueneToluene binding domain
Korea Soc-Assoc-Inst
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Critical Diseases Diagnostics Convergence Research Center > 1. Journal Articles
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