Development of novel on-line capillary gas chromatography-based analysis method for volatile organic compounds produced by aerobic fermentation

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Title
Development of novel on-line capillary gas chromatography-based analysis method for volatile organic compounds produced by aerobic fermentation
Author(s)
Hyeok Won LeeJung Ho Park; Hee-Suk Lee; Chun Sug Kim; Jin Gyeom LeeWon-Kyo KimKyoung Hwa RyuJungoh AhnEun Gyo Lee; S W Kim; J M Jeon; Y H Yang; Eui Sung Choi; Hong-Weon Lee
Bibliographic Citation
Journal of Bioscience and Bioengineering, vol. 127, no. 1, pp. 121-127
Publication Year
2019
Abstract
Many volatile compounds, such as isoprene, a precursor used in the synthesis of natural rubber, have been produced through fermentation using genetically engineered microorganisms. Despite this biotechnological success, measuring the concentrations of volatile compounds during fermentation is difficult because of their high volatility. In current systems, off-line analytical methods usually lead to product loss, whereas on-line methods raise the production cost due to the requirement of complex devices. Here, we developed a novel on-line gas chromatography (GC)-based system for analyzing the concentration of isoprene with the aim to minimize the cost and requirement for devices as compared to current strategies. In this system, a programmable logic controller is used to combine conventional GC with a syringe pump module (SPM) directly connected to the exhaust pipe of the fermentor, and isoprene-containing samples are continuously pumped from the SPM into the GC using an air cylinder recycle stream. We showed that this novel system enables isoprene analysis during fermentation with convenient equipment and without the requirement of an expensive desorption tube. Furthermore, this system may be extended to the detection of other volatile organic compounds in fermentation or chemical processes.
Keyword
Isoprene productionOn-line monitoringProgrammable logic controllerSyringe pump moduleIsoprene
ISSN
1389-1723
Publisher
Soc Bioscience Bioengineering Japan
DOI
http://dx.doi.org/10.1016/j.jbiosc.2018.07.007
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Division of Bio Technology Innovation > Bio-Evaluation Center > 1. Journal Articles
Division of Bio Technology Innovation > 1. Journal Articles
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