Effect of Veratrum maackii on testosterone propionate-induced benign prostatic hyperplasia in rats

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Title
Effect of Veratrum maackii on testosterone propionate-induced benign prostatic hyperplasia in rats
Author(s)
H S Park; C S Seo; C U B Wijerathne; H Y Jeong; Og Sung Moon; Young Won SeoYoung Suk Won; H Y Son; J H Lim; H J Kwun
Bibliographic Citation
Biological & Pharmaceutical Bulletin, vol. 42, no. 1, pp. 1-9
Publication Year
2019
Abstract
Veratrum maackii (VM), a perennial plant in the Melanthiaceae family, has anti-hypertensive, anti-cholinergic, anti-asthmatic, anti-tussive, anti-fungal, anti-melanogenesis, and anti-tumor activities. Here, we investigated the therapeutic effect of VM on benign prostatic hyperplasia (BPH) in human normal prostate cell line (WPMY-1) and a testosterone propionate-induced BPH animal model. WPMY-1 cells were treated with VM (1-10 μg/mL) and testosterone propionate (100nM). BPH in rats was generated via daily subcutaneous injections of testosterone propionate (3mg/kg) dissolved in corn oil, for 4 weeks. VM (150mg/kg) was administered daily for 4 weeks by oral gavage concurrently with the testosterone propionate. All rats were sacrificed and the prostates were dissected, weighed, and subjected to histological, immunohistochemical, and biochemical examinations. Immunoblotting experiments indicated that WPMY-1 cells treated testosterone propionate had increased expression of prostate specific antigen (PSA) and androgen receptor (AR), and treatment with VM or finasteride blocked this effect. In rat model, VM significantly reduced prostate weight, prostatic hyperplasia, prostatic levels of dihydrotestosterone (DHT), and expression of proliferation markers such as proliferating cell nuclear antigen (PCNA) and cyclin D1, but increased the expression of pro-apoptotic Bcl-2-associated X protein (Bax) and the cleavage of caspase-3. VM administration also suppressed the testosterone propionate-induced activation of nuclear factor-kappaB (NF-κB). Our results indicate that VM effectively represses the development of testosterone propionate-induced BPH, suggesting it may be a useful treatment agent for BPH.
Keyword
Veratrum maackiibenign prostatic hyperplasianuclear factor-kappaB (NF-κB)testosterone propionate
ISSN
0918-6158
Publisher
Pharmaceutical Soc Japan
DOI
http://dx.doi.org/10.1248/bpb.b18-00313
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Laboratory Animal Resource Center > 1. Journal Articles
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