Identification of MazF homologue in Legionella pneumophila which cleaves RNA at the AACU sequence

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Title
Identification of MazF homologue in Legionella pneumophila which cleaves RNA at the AACU sequence
Author(s)
M Shaku; Jung Ho Park; M Inouye; Y Yamaguchi
Bibliographic Citation
Journal of Molecular Microbiology and Biotechnology, vol. 28, no. 6, pp. 269-280
Publication Year
2018
Abstract
MazF is a sequence-specific endoribonuclease that is widely conserved in bacteria and archaea. Here, we found an MazF homologue (MazF-lp; LPO-p0114) in Legionella pneumophila. The mazF-lp gene overlaps 14 base pairs with the upstream gene mazE-lp (MazE-lp; LPO-p0115). The induction of mazF-lp caused cell growth arrest, while mazE-lp co-induction recovered cell growth in Escherichia coli. In vivo and in vitro primer extension experiments showed that MazF-lp is a sequence-specific endoribonuclease cleaving RNA at AACU. The endoribonuclease activity of purified MazF-lp was inhibited by purified MazE-lp. We found that MazE-lp and the MazEF-lp complex specifically bind to the palindromic sequence present in the 5'-untranslated region of the mazEF-lp operon. MazE-lp and MazEF-lp both likely function as a repressor for the mazEF-lp operon and for other genes, including icmR, whose gene product functions as a secretion chaperone for the IcmQ pore-forming protein, by specifically binding to the palindromic sequence in 5'-UTR of these genes.
Keyword
MazFToxinToxin-antitoxin system
ISSN
1464-1801
Publisher
Karger
DOI
http://dx.doi.org/10.1159/000497146
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Bio-Evaluation Center > 1. Journal Articles
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