Protein tyrosine phosphatase, receptor type B (PTPRB) inhibits brown adipocyte differentiation through regulation of VEGFR2 phosphorylation

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Title
Protein tyrosine phosphatase, receptor type B (PTPRB) inhibits brown adipocyte differentiation through regulation of VEGFR2 phosphorylation
Author(s)
Ji Soo Kim; Won Kon KimKyoung Jin OhEun Woo LeeBaek Soo HanSang Chul LeeKwang-Hee Bae
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 29, no. 4, pp. 645-650
Publication Year
2019
Abstract
Brown adipocytes have an important role in the regulation of energy balance through uncoupling protein-1 (UCP-1)-mediated nonshivering thermogenesis. Although brown adipocytes have been highlighted as a new therapeutic target for the treatment of metabolic diseases, such as obesity and type II diabetes in adult humans, the molecular mechanism underlying brown adipogenesis is not fully understood. We recently found that protein tyrosine phosphatase receptor type B (PTPRB) expression dramatically decreased during brown adipogenic differentiation. In this study, we investigated the functional roles of PTPRB and its regulatory mechanism during brown adipocyte differentiation. Ectopic expression of PTPRB led to a reduced brown adipocyte differentiation by suppressing the tyrosine phosphorylation of VEGFR2, whereas a catalytic inactive PTPRB mutant showed no effects on differentiation and phosphorylation. Consistently, the expression of brown adipocyte-related genes, such as UCP-1, PGC-1α, PRDM16, PPAR-γ, and CIDEA, were significantly inhibited by PTPRB overexpression. Overall, these results suggest that PTPRB functions as a negative regulator of brown adipocyte differentiation through its phosphatase activity-dependent mechanism and may be used as a target protein for the regulation of obesity and type II diabetes.
Keyword
Brown adipogenesisobesityPTPRBVEGFR2
ISSN
1017-7825
Publisher
South Korea
DOI
http://dx.doi.org/10.4014/jmb.1810.10033
Type
Article
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Division of Research on National Challenges > Biodefense Research Center > 1. Journal Articles
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