Derrone induces autophagic cell death through induction of ROS and ERK in A549 cells

Cited 8 time in scopus
Metadata Downloads
Derrone induces autophagic cell death through induction of ROS and ERK in A549 cells
Myung-Ji Kang; Soo Yeon Kim; Eun-Bin Kwon; Yang Hee Jo; M K Lee; Hyun Sun Lee; D O Moon; Mun-Ock Kim
Bibliographic Citation
PLoS One, vol. 14, no. 6, pp. e0218659-e0218659
Publication Year
We studied the effect of derrone (DR), one of the major compounds of unripe fruits of Cudrania tricuspidata, on cancer cell death. DR inhibited cell growth of various cancer cells, and that was partially associated with apoptosis in A549 cells. DR showed the autophagic features, such as the conversion of LC3-I to LC3-II, the formation of autophagosome and the downregulation of SQSTM1/p62 (p62). The treatment of autophagy inhibitor reversed DRmediated cell death, suggesting that DR induces autophagic cell death. The increase of cytoplasmic Ca2+ and ROS by DR treatment significantly influences the formation of autophagosomes; however, only ROS scavengers significantly rescued the reduced cell viability. Additional results revealed that treatment of DR induces sustained phosphorylation of ERK and the inhibition of ERK phosphorylation using U0126 (ERK inhibitor) markedly attenuated DR-induced cell death. Overall, these results suggest that DR induces autophagic cell death through intracellular ROS and sustained ERK phosphorylation in A549 cells.
Public Library of Science
Appears in Collections:
Ochang Branch Institute > Natural Product Research Center > 1. Journal Articles
Files in This Item:

Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.