Comparative evaluation of hormones and hormone-like molecule in lineage specification of human induced pluripotent stem cells

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dc.contributor.authorSeon A Choi-
dc.contributor.authorJu-Hyun An-
dc.contributor.authorSeung Hwan Lee-
dc.contributor.authorGeun-Hui Lee-
dc.contributor.authorHae Jun Yang-
dc.contributor.authorPil Soo Jeong-
dc.contributor.authorJae Jin Cha-
dc.contributor.authorSanghoon Lee-
dc.contributor.authorYoung-Ho Park-
dc.contributor.authorBong-Seok Song-
dc.contributor.authorBo Woong Sim-
dc.contributor.authorYoung-Hyun Kim-
dc.contributor.authorJi-Su Kim-
dc.contributor.authorYeung Bae Jin-
dc.contributor.authorJae Won Huh-
dc.contributor.authorSang-Rae Lee-
dc.contributor.authorJong Hee Lee-
dc.contributor.authorSun-Uk Kim-
dc.date.accessioned2019-10-28T16:30:17Z-
dc.date.available2019-10-28T16:30:17Z-
dc.date.issued2019-
dc.identifier.issn20053606-
dc.identifier.uri10.15283/ijsc18137ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/18905-
dc.description.abstractBACKGROUND AND OBJECTIVES: Proficient differentiation of human pluripotent stem cells (hPSCs) into specific lineages is required for applications in regenerative medicine. A growing amount of evidences had implicated hormones and hormone-like molecules as critical regulators of proliferation and lineage specification during in vivo development. Therefore, a deeper understanding of the hormones and hormone-like molecules involved in cell fate decisions is critical for efficient and controlled differentiation of hPSCs into specific lineages. Thus, we functionally and quantitatively compared the effects of diverse hormones (estradiol 17-β (E2), progesterone (P4), and dexamethasone (DM)) and a hormone-like molecule (retinoic acid (RA)) on the regulation of hematopoietic and neural lineage specification. METHODS AND RESULTS: We used 10 nM E2, 3 μM P4, 10 nM DM, and 10 nM RA based on their functional in vivo developmental potential. The sex hormone E2 enhanced functional activity of hematopoietic progenitors compared to P4 and DM, whereas RA impaired hematopoietic differentiation. In addition, E2 increased CD34+CD45+ cells with progenitor functions, even in the CD43- population, a well-known hemogenic marker. RA exhibited lineage-biased potential, preferentially committing hPSCs toward the neural lineage while restricting the hematopoietic fate decision. CONCLUSIONS: Our findings reveal unique cell fate potentials of E2 and RA treatment and provide valuable differentiation information that is essential for hPSC applications.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleComparative evaluation of hormones and hormone-like molecule in lineage specification of human induced pluripotent stem cells-
dc.title.alternativeComparative evaluation of hormones and hormone-like molecule in lineage specification of human induced pluripotent stem cells-
dc.typeArticle-
dc.citation.titleInternational Journal of Stem Cells-
dc.citation.number2-
dc.citation.endPage250-
dc.citation.startPage240-
dc.citation.volume12-
dc.contributor.affiliatedAuthorSeon A Choi-
dc.contributor.affiliatedAuthorJu-Hyun An-
dc.contributor.affiliatedAuthorSeung Hwan Lee-
dc.contributor.affiliatedAuthorGeun-Hui Lee-
dc.contributor.affiliatedAuthorHae Jun Yang-
dc.contributor.affiliatedAuthorPil Soo Jeong-
dc.contributor.affiliatedAuthorJae Jin Cha-
dc.contributor.affiliatedAuthorSanghoon Lee-
dc.contributor.affiliatedAuthorYoung-Ho Park-
dc.contributor.affiliatedAuthorBong-Seok Song-
dc.contributor.affiliatedAuthorBo Woong Sim-
dc.contributor.affiliatedAuthorYoung-Hyun Kim-
dc.contributor.affiliatedAuthorJi-Su Kim-
dc.contributor.affiliatedAuthorYeung Bae Jin-
dc.contributor.affiliatedAuthorJae Won Huh-
dc.contributor.affiliatedAuthorSang-Rae Lee-
dc.contributor.affiliatedAuthorJong Hee Lee-
dc.contributor.affiliatedAuthorSun-Uk Kim-
dc.contributor.alternativeName최선아-
dc.contributor.alternativeName안주현-
dc.contributor.alternativeName이승환-
dc.contributor.alternativeName이근희-
dc.contributor.alternativeName양해준-
dc.contributor.alternativeName정필수-
dc.contributor.alternativeName차재진-
dc.contributor.alternativeName이상훈-
dc.contributor.alternativeName박영호-
dc.contributor.alternativeName송봉석-
dc.contributor.alternativeName심보웅-
dc.contributor.alternativeName김영현-
dc.contributor.alternativeName김지수-
dc.contributor.alternativeName진영배-
dc.contributor.alternativeName허재원-
dc.contributor.alternativeName이상래-
dc.contributor.alternativeName이종희-
dc.contributor.alternativeName김선욱-
dc.identifier.bibliographicCitationInternational Journal of Stem Cells, vol. 12, no. 2, pp. 240-250-
dc.identifier.doi10.15283/ijsc18137-
dc.subject.keywordCell fate decision-
dc.subject.keywordEstradiol-17β-
dc.subject.keywordHematopoietic differentiation-
dc.subject.keywordHuman induced pluripotent stem cells-
dc.subject.keywordRetinoic acid-
dc.subject.keywordlineage specification-
dc.subject.localCell fate decision-
dc.subject.localEstradiol-17β-
dc.subject.localHematopoietic differentiation-
dc.subject.localHuman induced pluripotent stem cells-
dc.subject.localHuman induced pluripotent stem cell-
dc.subject.localRetinoic acid-
dc.subject.locallineage specification-
dc.description.journalClassY-
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > National Primate Research Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Primate Resources Center > 1. Journal Articles
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