Comparative evaluation of hormones and hormone-like molecule in lineage specification of human induced pluripotent stem cells

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Title
Comparative evaluation of hormones and hormone-like molecule in lineage specification of human induced pluripotent stem cells
Author(s)
Seon A Choi; Ju-Hyun An; Seung Hwan Lee; Geun-Hui Lee; Hae Jun Yang; Pil Soo Jeong; Jae Jin Cha; Sanghoon Lee; Young-Ho ParkBong-Seok SongBo Woong SimYoung-Hyun KimJi-Su Kim; Yeung Bae Jin; Jae Won Huh; Sang-Rae Lee; Jong Hee LeeSun-Uk Kim
Bibliographic Citation
International Journal of Stem Cells, vol. 12, no. 2, pp. 240-250
Publication Year
2019
Abstract
BACKGROUND AND OBJECTIVES: Proficient differentiation of human pluripotent stem cells (hPSCs) into specific lineages is required for applications in regenerative medicine. A growing amount of evidences had implicated hormones and hormone-like molecules as critical regulators of proliferation and lineage specification during in vivo development. Therefore, a deeper understanding of the hormones and hormone-like molecules involved in cell fate decisions is critical for efficient and controlled differentiation of hPSCs into specific lineages. Thus, we functionally and quantitatively compared the effects of diverse hormones (estradiol 17-β (E2), progesterone (P4), and dexamethasone (DM)) and a hormone-like molecule (retinoic acid (RA)) on the regulation of hematopoietic and neural lineage specification. METHODS AND RESULTS: We used 10 nM E2, 3 μM P4, 10 nM DM, and 10 nM RA based on their functional in vivo developmental potential. The sex hormone E2 enhanced functional activity of hematopoietic progenitors compared to P4 and DM, whereas RA impaired hematopoietic differentiation. In addition, E2 increased CD34+CD45+ cells with progenitor functions, even in the CD43- population, a well-known hemogenic marker. RA exhibited lineage-biased potential, preferentially committing hPSCs toward the neural lineage while restricting the hematopoietic fate decision. CONCLUSIONS: Our findings reveal unique cell fate potentials of E2 and RA treatment and provide valuable differentiation information that is essential for hPSC applications.
Keyword
Cell fate decisionEstradiol-17βHematopoietic differentiationHuman induced pluripotent stem cellsRetinoic acidlineage specification
ISSN
2005-3606
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.15283/ijsc18137
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > National Primate Research Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Primate Resources Center > 1. Journal Articles
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