Derivation of primitive neural stem cells from human-induced pluripotent stem cells

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dc.contributor.authorW J Shin-
dc.contributor.authorJ H Seo-
dc.contributor.authorH W Choi-
dc.contributor.authorY J Hong-
dc.contributor.authorW J Lee-
dc.contributor.authorJ I Chae-
dc.contributor.authorS J Kim-
dc.contributor.authorJeong Woong Lee-
dc.contributor.authorK Hong-
dc.contributor.authorH Song-
dc.contributor.authorC Park-
dc.contributor.authorJ T Do-
dc.date.accessioned2020-02-07T16:30:13Z-
dc.date.available2020-02-07T16:30:13Z-
dc.date.issued2019-
dc.identifier.issn00219967-
dc.identifier.uri10.1002/cne.24727ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/19072-
dc.description.abstractHuman-induced pluripotent stem cells (hiPSCs) have facilitated studies on organ development and differentiation into specific lineages in in vitro systems. Although numerous studies have focused on cellular differentiation into neural lineage using hPSCs, most studies have initially evaluated embryoid body (EB) formation, eventually yielding terminally differentiated neurons with limited proliferation potential. This study aimed to establish human primitive neural stem cells (pNSCs) from exogene-free hiPSCs without EB formation. To derive pNSCs, we optimized N2B27 neural differentiation medium through supplementation of two inhibitors, CHIR99021 (GSK-3 inhibitor) and PD0325901 (MEK inhibitor), and growth factors including basic fibroblast growth factor (bFGF) and human leukemia inhibitory factor (hLIF). Consequently, pNSCs were efficiently derived and cultured over a long term. pNSCs displayed differentiation potential into neurons, astrocytes, and oligodendrocytes. These early NSC types potentially promote the clinical application of hiPSCs to cure human neurological disorders.-
dc.publisherWiley-
dc.titleDerivation of primitive neural stem cells from human-induced pluripotent stem cells-
dc.title.alternativeDerivation of primitive neural stem cells from human-induced pluripotent stem cells-
dc.typeArticle-
dc.citation.titleJournal of Comparative Neurology-
dc.citation.number18-
dc.citation.endPage3033-
dc.citation.startPage3023-
dc.citation.volume527-
dc.contributor.affiliatedAuthorJeong Woong Lee-
dc.contributor.alternativeName신우정-
dc.contributor.alternativeName서지혜-
dc.contributor.alternativeName최현우-
dc.contributor.alternativeName홍연주-
dc.contributor.alternativeName이원지-
dc.contributor.alternativeName채정일-
dc.contributor.alternativeName김성주-
dc.contributor.alternativeName이정웅-
dc.contributor.alternativeName홍권호-
dc.contributor.alternativeName송혁-
dc.contributor.alternativeName박찬규-
dc.contributor.alternativeName도정태-
dc.identifier.bibliographicCitationJournal of Comparative Neurology, vol. 527, no. 18, pp. 3023-3033-
dc.identifier.doi10.1002/cne.24727-
dc.subject.keyworddifferentiation-
dc.subject.keywordhuman-induced pluripotent stem cells-
dc.subject.keywordneural lineage-
dc.subject.keywordprimitive neural stem cells-
dc.subject.localdifferentiation-
dc.subject.localDifferentiation-
dc.subject.localhuman-induced pluripotent stem cells-
dc.subject.localneural lineage-
dc.subject.localprimitive neural stem cells-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
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