Derivation of primitive neural stem cells from human-induced pluripotent stem cells

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Title
Derivation of primitive neural stem cells from human-induced pluripotent stem cells
Author(s)
W J Shin; J H Seo; H W Choi; Y J Hong; W J Lee; J I Chae; S J Kim; Jeong Woong Lee; K Hong; H Song; C Park; J T Do
Bibliographic Citation
Journal of Comparative Neurology, vol. 527, no. 18, pp. 3023-3033
Publication Year
2019
Abstract
Human-induced pluripotent stem cells (hiPSCs) have facilitated studies on organ development and differentiation into specific lineages in in vitro systems. Although numerous studies have focused on cellular differentiation into neural lineage using hPSCs, most studies have initially evaluated embryoid body (EB) formation, eventually yielding terminally differentiated neurons with limited proliferation potential. This study aimed to establish human primitive neural stem cells (pNSCs) from exogene-free hiPSCs without EB formation. To derive pNSCs, we optimized N2B27 neural differentiation medium through supplementation of two inhibitors, CHIR99021 (GSK-3 inhibitor) and PD0325901 (MEK inhibitor), and growth factors including basic fibroblast growth factor (bFGF) and human leukemia inhibitory factor (hLIF). Consequently, pNSCs were efficiently derived and cultured over a long term. pNSCs displayed differentiation potential into neurons, astrocytes, and oligodendrocytes. These early NSC types potentially promote the clinical application of hiPSCs to cure human neurological disorders.
Keyword
differentiationhuman-induced pluripotent stem cellsneural lineageprimitive neural stem cells
ISSN
0021-9967
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/cne.24727
Type
Article
Appears in Collections:
Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
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