Embryo aggregation regulates in vitro stress conditions to promote developmental competence in pigs

Cited 2 time in scopus
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Title
Embryo aggregation regulates in vitro stress conditions to promote developmental competence in pigs
Author(s)
Pil Soo Jeong; Seung-Bin Yoon; Mun-Hyeong Lee; Hee Chang SonHwal Yong Lee; Sanghoon Lee; Bon Sang KooKang Jin JeongJong Hee Lee; Yeung Bae Jin; Bong-Seok SongJi-Su KimSun-Uk Kim; D B Koo; Bo Woong Sim
Bibliographic Citation
PeerJ, vol. 7, pp. e8143-e8143
Publication Year
2019
Abstract
Embryo aggregation is a useful method to produce blastocysts with high developmental competence to generate more offspring in various mammals, but the underlying mechanism(s) regarding the beneficial effects are largely unknown. In this study, we investigated the effects of embryo aggregation using 4-cell stage embryos in in vitro developmental competence and the relationship of stress conditions in porcine early embryogenesis. We conducted aggregation using the well of the well system and confirmed that aggregation using two or three embryos was useful for obtaining blastocysts. Aggregated embryos significantly improved developmental competence, including blastocyst formation rate, blastomere number, ICM/TE ratio, and cellular survival rate, compared to non-aggregated embryos. Investigation into the relationship between embryo aggregation and stress conditions revealed that mitochondrial function increased, and oxidative and endoplasmic reticulum (ER)-stress decreased compared to 1X (non-aggregated embryos) blastocysts. In addition, 3X (three-embryo aggregated) blastocysts increased the expression of pluripotency, anti-apoptosis, and implantation related genes, and decreased expression of pro-apoptosis related genes. Therefore, these findings indicate that embryo aggregation regulates in vitro stress conditions to increase developmental competence and contributes to the in vitro production of high-quality embryos and the large-scale production of transgenic and chimeric pigs.
Keyword
Blastocyst qualityEndoplasmic reticulum stressMitochondrial functionPig embryo aggregationReactive oxygen species
ISSN
2167-8359
Publisher
PeerJ Inc
DOI
http://dx.doi.org/10.7717/peerj.8143
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Primate Resources Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > National Primate Research Center > 1. Journal Articles
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