Identification of a novel imprinted transcript in the porcine GNAS complex locus using methylome and transcriptome of parthenogenetic fetuses

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Title
Identification of a novel imprinted transcript in the porcine GNAS complex locus using methylome and transcriptome of parthenogenetic fetuses
Author(s)
J Ahn; H Wu; J Lee; I S Hwang; D Yu; Jin Seop Ahn; Jeong Woong LeeS Hwang; K Lee
Bibliographic Citation
Genes, vol. 11, pp. 96-96
Publication Year
2020
Abstract
Genomic imprinting in domestic animals contributes to the variance of performance traits. However, research remains to be done on large-scale detection of epigenetic landscape of porcine imprinted loci including the GNAS complex locus. The purpose of this study was to generate porcine parthenogenetic fetuses and comprehensively identify imprinting patterns of the GNAS locus in transcript levels. To this end, both normally fertilized and bimaternal (uniparental) parthenogenetic porcine fetuses were generated, and whole genome bisulfite sequencing (WGBS) and RNA sequencing (RNA-seq) were performed to construct methylome and transcriptome, respectively. Differentially methylated regions (DMRs) between the fetuses were identified through methylome analysis, and parental-origin-specific expression patterns of transcripts were examined with transcriptome. As a result, three major DMRs were identified: paternally methylated Nesp DMR, maternally methylated Nespas-Gnasxl DMR, and maternally methylated Exon1B-Exon1A DMR. Parental-origin-specific expressions of those five DMR-affected transcripts were found, including a novel imprinted transcript, Exon1B, in pigs. In conclusion, using parthenotes, parental-origin-specific imprinting patterns in the porcine GNAS locus was comprehensively identified, and our approach paves the way for the discovery of novel imprinted genes and loci in a genomic context across species.
Keyword
GNASRNA-sequencingdifferentially methylated regionfetusgenomic imprintingparthenogeneticparthenoteporcinewhole genome bisulfite sequencing
ISSN
2073-4425
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/genes11010096
Type
Article
Appears in Collections:
Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
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