CRM646-A, a fungal metabolite, induces nucleus condensation by increasing Ca2+ levels in Rat 3Y1 fibroblast cells

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Title
CRM646-A, a fungal metabolite, induces nucleus condensation by increasing Ca2+ levels in Rat 3Y1 fibroblast cells
Author(s)
Y Asami; Sun Ok Kim; Jun-Pil JangSung-Kyun KoBo Yeon Kim; H Osada; Jae-Hyuk JangJong Seog Ahn
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 30, no. 1, pp. 31-37
Publication Year
2020
Abstract
We previously identified a new heparinase inhibitor fungal metabolite, named CRM646-A, which showed inhibition of heparinase and telomerase activities in an in vitro enzyme assay and antimetastatic activity in a cell-based assay. In this study, we elucidated the mechanism by which CRM646-A rapidly induced nucleus condensation, plasma membrane disruption and morphological changes by increasing intracellular Ca2+ levels. Furthermore, PD98059, a mitogen-activated protein kinase (MEK) inhibitor, inhibited CRM646-A-induced nucleus condensation through ERK1/2 activation in rat 3Y1 fibroblast cells. We identified CRM646-A as a Ca2+ ionophore-like agent with a distinctly different chemical structure from that of previously reported Ca2+ ionophores. These results indicate that CRM646-A has the potential to be used as a new and effective antimetastatic drug.
Keyword
Ca² ionophore-like agentCa² signalingERK pathwayNucleus condensationplasma membrane
ISSN
1017-7825
Publisher
South Korea
DOI
http://dx.doi.org/10.4014/jmb.1908.08043
Type
Article
Appears in Collections:
Ochang Branch Institute > Anticancer Agent Research Center > 1. Journal Articles
Ochang Branch Institute > Natural Medicine Research Center > 1. Journal Articles
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