Spiraea prunifolia var. simpliciflora attenuates oxidative stress and inflammatory responses in a murine model of lipopolysaccharide-iInduced acute lung injury and TNF-α-stimulated NCI-H292 cells

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Title
Spiraea prunifolia var. simpliciflora attenuates oxidative stress and inflammatory responses in a murine model of lipopolysaccharide-iInduced acute lung injury and TNF-α-stimulated NCI-H292 cells
Author(s)
Ba Wool Lee; Ji Hye Ha; Han-Gyo Shin; Seong Hun Jeong; Da-Bin Jeon; Ju-Hong Kim; Ji Young ParkHyung Jun KwonKyungsook JungWoo Song Lee; H Y Kim; S H Kim; Hyun Jae JangYoung Bae RyuIn Chul Lee
Bibliographic Citation
Antioxidants, vol. 9, pp. 198-198
Publication Year
2020
Abstract
Spiraea prunifolia var. simpliciflora (SP) is traditionally used as an herbal remedy to treat fever, malaria, and emesis. This study aimed to evaluate the anti-oxidative and anti-inflammatory properties of the methanol extract of SP leaves in tumor necrosis factor (TNF)-α-stimulated NCI-H292 cells and in a lipopolysaccharide (LPS)-induced acute lung injury (ALI) mouse model. SP decreased the number of inflammatory cells and the levels of TNF-α, interleukin (IL)-1β, and IL-6 in the bronchoalveolar lavage fluid, and inflammatory cell infiltration in the lung tissues of SP-treated mice. In addition, SP significantly suppressed the mRNA and protein levels of TNF-α, IL-1β, and IL-6 in TNF-α-stimulated NCI-H292 cells. SP significantly suppressed the phosphorylation of the mitogen-activated protein kinases (MAPKs) and p65-nuclear factor-kappa B (NF-κB) in LPS-induced ALI mice and TNF-α-stimulated NCI-H292 cells. SP treatment enhanced the nuclear translocation of nuclear factor erythroid 2-related factor (Nrf2) with upregulated antioxidant enzymes and suppressed reactive oxygen species (ROS)-mediated oxidative stress in the lung tissues of LPS-induced ALI model and TNF-α-stimulated NCI-H292 cells. Collectively, SP effectively inhibited airway inflammation and ROS-mediated oxidative stress, which was closely related to its ability to induce activation of Nrf2 and inhibit the phosphorylation of MAPKs and NF-κB. These findings suggest that SP has therapeutic potential for the treatment of ALI.
Keyword
Acute lung injuryAnti-oxidant activityNuclear factor erythroid 2-related factorNuclear factor-kappaBSimplicifloraSpiraea prunifolia var
ISSN
2076-3921
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/antiox9030198
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
Ochang Branch Institute > Natural Medicine Research Center > 1. Journal Articles
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