Identification and genome analysis of Vibrio coralliilyticus causing mortality of Pacific oyster (Crassostrea gigas) larvae

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Title
Identification and genome analysis of Vibrio coralliilyticus causing mortality of Pacific oyster (Crassostrea gigas) larvae
Author(s)
H J Kim; J W Jun; S S Giri; C Chi; S Yun; S G Kim; S W Kim; S J Han; J Kwon; O T Oh; S B Lee; Ji Hyung Kim; S C Park
Bibliographic Citation
Pathogens, vol. 9, no. 3, pp. 206-206
Publication Year
2020
Abstract
Vibrio coralliilyticus is known as a coral pathogen that also infects marine bivalve larvae worldwide. It is considered to be one of the major constraints in artificial marine bivalve seed production as it causes mortality. In this study, we first isolated and characterized a high virulent of V. coralliilyticus designated as SNUTY-1 that was the cause of Pacific oyster larvae mortality in Korea. In the pathogenicity test, exposure to 2.14 × 105 CFU/mL for 24 h caused mortality to 88.65 ± 2.4% of the tested healthy Pacific oyster larvae. SNUTY-1 showed anti-microbial resistance to β-lactams, such as penicillins, cephalosporins, and carbapenems. We sequenced and assembled the complete genome of SNUTY-1 (5,842,676 bp), consisting of two chromosomes (Chr I and Chr II) and two plasmids (pSNUTY1 and pSNUTY2). The COG functional analysis confirmed that Chr I had more genes associated with basic cellular functions in comparison to Chr II. The results of the phylogenetic trees based on OrthoANI values indicated that the SNUTY-1 was closely related to V. coralliilyticus strains. SNUTY-1 had a unique plasmid (pSNUTY2), which could mean that the Korean isolate is different from other sequenced V. coralliilyticus strains from different geographical origins. Toxic proteins such as cytolysin/hemolysin and extracellular metalloprotease genes were encoded on Chr I and Chr II of SNUTY-1. These data facilitate the control of V. coralliilyticus infections in aquaculture by providing valuable insights into the biodiversity of this organism and valuable information for the study of virulence factors.
Keyword
ChrIChrIIPacific oyster (Crassostrea gigas) larvaeSNUTY-1Vibrio speciesgenome analyses
ISSN
2076-0817
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/pathogens9030206
Type
Article
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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