Acclimation of bacterial cell state for high-throughput enzyme engineering using a DmpR-dependent transcriptional activation system = 미생물 세포 상태 순응과 페놀 감지 전사 조절인자를 이용한 초고속 효소 개량

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dc.contributor.authorKil Koang Kwon-
dc.contributor.authorSoo Jin Yeom-
dc.contributor.authorSu-Lim Choi-
dc.contributor.authorEugene Rha-
dc.contributor.authorHyewon Lee-
dc.contributor.authorHaseong Kim-
dc.contributor.authorDae-Hee Lee-
dc.contributor.authorSeung Goo Lee-
dc.date.accessioned2020-04-24T16:30:36Z-
dc.date.available2020-04-24T16:30:36Z-
dc.date.issued2020-
dc.identifier.issn2045-2322-
dc.identifier.uri10.1038/s41598-020-62892-1ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/19444-
dc.description.abstractGenetic circuit-based biosensors have emerged as an effective analytical tool in synthetic biology; these biosensors can be applied to high-throughput screening of new biocatalysts and metabolic pathways. Sigma 54 (σ54)-dependent transcription factor (TF) can be a valuable component of these biosensors owing to its intrinsic silent property compared to most of the housekeeping sigma 70 (σ70) TFs. Here, we show that these unique characteristics of σ54-dependent TFs can be used to control the host cell state to be more appropriate for high-throughput screening. The acclimation of cell state was achieved by using guanosine (penta)tetraphosphate ((p)ppGpp)-related genes (relA, spoT) and nutrient conditions, to link the σ54 TF-based reporter expression with the target enzyme activity. By controlling stringent programmed responses and optimizing assay conditions, catalytically improved tyrosine phenol lyase (TPL) enzymes were successfully obtained using a σ54-dependent DmpR as the TF component, demonstrating the practical feasibility of this biosensor. This combinatorial strategy of biosensors using σ factor-dependent TFs will allow for more effective high-throughput enzyme engineering with broad applicability.-
dc.publisherSpringer-Nature Pub Group-
dc.titleAcclimation of bacterial cell state for high-throughput enzyme engineering using a DmpR-dependent transcriptional activation system = 미생물 세포 상태 순응과 페놀 감지 전사 조절인자를 이용한 초고속 효소 개량-
dc.title.alternativeAcclimation of bacterial cell state for high-throughput enzyme engineering using a DmpR-dependent transcriptional activation system-
dc.typeArticle-
dc.citation.titleScientific Reports-
dc.citation.number0-
dc.citation.endPage6091-
dc.citation.startPage6091-
dc.citation.volume10-
dc.contributor.affiliatedAuthorKil Koang Kwon-
dc.contributor.affiliatedAuthorSoo Jin Yeom-
dc.contributor.affiliatedAuthorSu-Lim Choi-
dc.contributor.affiliatedAuthorEugene Rha-
dc.contributor.affiliatedAuthorHyewon Lee-
dc.contributor.affiliatedAuthorHaseong Kim-
dc.contributor.affiliatedAuthorDae-Hee Lee-
dc.contributor.affiliatedAuthorSeung Goo Lee-
dc.contributor.alternativeName권길광-
dc.contributor.alternativeName염수진-
dc.contributor.alternativeName최수림-
dc.contributor.alternativeName나유진-
dc.contributor.alternativeName이혜원-
dc.contributor.alternativeName김하성-
dc.contributor.alternativeName이대희-
dc.contributor.alternativeName이승구-
dc.identifier.bibliographicCitationScientific Reports, vol. 10, pp. 6091-6091-
dc.identifier.doi10.1038/s41598-020-62892-1-
dc.description.journalClassY-
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > 1. Journal Articles
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