DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kil Koang Kwon | - |
dc.contributor.author | Soo Jin Yeom | - |
dc.contributor.author | Su-Lim Choi | - |
dc.contributor.author | Eugene Rha | - |
dc.contributor.author | Hyewon Lee | - |
dc.contributor.author | Haseong Kim | - |
dc.contributor.author | Dae-Hee Lee | - |
dc.contributor.author | Seung Goo Lee | - |
dc.date.accessioned | 2020-04-24T16:30:36Z | - |
dc.date.available | 2020-04-24T16:30:36Z | - |
dc.date.issued | 2020 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | 10.1038/s41598-020-62892-1 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/19444 | - |
dc.description.abstract | Genetic circuit-based biosensors have emerged as an effective analytical tool in synthetic biology; these biosensors can be applied to high-throughput screening of new biocatalysts and metabolic pathways. Sigma 54 (σ54)-dependent transcription factor (TF) can be a valuable component of these biosensors owing to its intrinsic silent property compared to most of the housekeeping sigma 70 (σ70) TFs. Here, we show that these unique characteristics of σ54-dependent TFs can be used to control the host cell state to be more appropriate for high-throughput screening. The acclimation of cell state was achieved by using guanosine (penta)tetraphosphate ((p)ppGpp)-related genes (relA, spoT) and nutrient conditions, to link the σ54 TF-based reporter expression with the target enzyme activity. By controlling stringent programmed responses and optimizing assay conditions, catalytically improved tyrosine phenol lyase (TPL) enzymes were successfully obtained using a σ54-dependent DmpR as the TF component, demonstrating the practical feasibility of this biosensor. This combinatorial strategy of biosensors using σ factor-dependent TFs will allow for more effective high-throughput enzyme engineering with broad applicability. | - |
dc.publisher | Springer-Nature Pub Group | - |
dc.title | Acclimation of bacterial cell state for high-throughput enzyme engineering using a DmpR-dependent transcriptional activation system = 미생물 세포 상태 순응과 페놀 감지 전사 조절인자를 이용한 초고속 효소 개량 | - |
dc.title.alternative | Acclimation of bacterial cell state for high-throughput enzyme engineering using a DmpR-dependent transcriptional activation system | - |
dc.type | Article | - |
dc.citation.title | Scientific Reports | - |
dc.citation.number | 0 | - |
dc.citation.endPage | 6091 | - |
dc.citation.startPage | 6091 | - |
dc.citation.volume | 10 | - |
dc.contributor.affiliatedAuthor | Kil Koang Kwon | - |
dc.contributor.affiliatedAuthor | Soo Jin Yeom | - |
dc.contributor.affiliatedAuthor | Su-Lim Choi | - |
dc.contributor.affiliatedAuthor | Eugene Rha | - |
dc.contributor.affiliatedAuthor | Hyewon Lee | - |
dc.contributor.affiliatedAuthor | Haseong Kim | - |
dc.contributor.affiliatedAuthor | Dae-Hee Lee | - |
dc.contributor.affiliatedAuthor | Seung Goo Lee | - |
dc.contributor.alternativeName | 권길광 | - |
dc.contributor.alternativeName | 염수진 | - |
dc.contributor.alternativeName | 최수림 | - |
dc.contributor.alternativeName | 나유진 | - |
dc.contributor.alternativeName | 이혜원 | - |
dc.contributor.alternativeName | 김하성 | - |
dc.contributor.alternativeName | 이대희 | - |
dc.contributor.alternativeName | 이승구 | - |
dc.identifier.bibliographicCitation | Scientific Reports, vol. 10, pp. 6091-6091 | - |
dc.identifier.doi | 10.1038/s41598-020-62892-1 | - |
dc.description.journalClass | Y | - |
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