Troponin Aptamer on an Atomically Flat Au Nanoplate Platform for Detection of Cardiac Troponin I

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Title
Troponin Aptamer on an Atomically Flat Au Nanoplate Platform for Detection of Cardiac Troponin I
Author(s)
H Lee; H Youn; Ahreum Hwang; H Lee; J Y Park; W Kim; Y Yoo; C Ban; Taejoon Kang; B Kim
Bibliographic Citation
Nanomaterials, vol. 10, no. 7, pp. 1402-1402
Publication Year
2020
Abstract
Well-ordered bioreceptors on atomically flat Au surfaces can be a high-performance biosensor. Cardiac troponin I proteins (cTnIs) have been regarded as a specific biomarker for acute myocardial infarction (AMI). Here, we report the accurate detection of cTnIs using an aptamer-immobilized Au nanoplate platform. The single-crystalline and atomically flat Au nanoplate was characterized by atomic force microscopy. For the precise detection of cTnI, we immobilized an aptamer that can strongly bind to cTnI onto an atomically flat Au nanoplate. Using the aptamer-immobilized Au nanoplate, cTnIs were successfully detected at a concentration of 100 aM (2.4 fg/mL) in buffer solution. Furthermore, cTnIs in serum could be identified at a concentration of 100 fM (2.4 pg/mL). The total assay time was ~7 h. Importantly, the aptamer-immobilized Au nanoplate enabled us to diagnose AMI patients accurately, suggesting the potential of the present method in the diagnosis of AMI.
Keyword
cTnIAptamerNanoplateSurface-enhanced Raman scattering
ISSN
2079-4991
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/nano10071402
Type
Article
Appears in Collections:
Division of Biomaterials Research > Bionanotechnology Research Center > 1. Journal Articles
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