Croton hirtus L'Her extract prevents inflammation in RAW264.7 macrophages via inhibition of NF-κB signaling pathway

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Title
Croton hirtus L'Her extract prevents inflammation in RAW264.7 macrophages via inhibition of NF-κB signaling pathway
Author(s)
M J Kim; J G Kim; K M Sydara; Sang Woo Lee; S K Jung
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 30, no. 4, pp. 490-496
Publication Year
2020
Abstract
Consumption of anti-inflammatory nutraceuticals may help treat or prevent inflammation-related illnesses such as diabetes, cardiovascular disease, and cancer. This study evaluated the effect of Croton hirtus L'Her extract (CHE) on lipopolysaccharide (LPS)-induced nitric oxide (NO) production and nuclear factor kappa-B (NF-κB) signaling cascades. CHE significantly suppressed LPS-induced NO production and inducible nitric oxide synthase (iNOS) expression in RAW264.7 macrophages, although cyclooxygenase (COX)-2 expression was not affected. CHE also suppressed LPS-induced IκB kinase (IKK), IκB, and p65 phosphorylation in RAW264.7 cells. Western blot and immunofluorescence assays of cytosol and nuclear p65 and the catalytic subunit of NF-κB showed that CHE suppressed LPS-induced p65 translocation from the cytosol to the nucleus. CHE also suppressed LPS-induced Interleukin (IL)-6 and tumor necrosis factor (TNF)-α production in RAW264.7 cells. These results suggest that CHE prevents NO-mediated inflammation by suppressing NF-κB and inflammatory cytokines.
Keyword
Croton hirtus L'HerInflammationMacrophageNF-κBNitric oxideNutraceuticals
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.4014/JMB.1908.08045
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > International Biological Material Research Center > 1. Journal Articles
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