Anti-allergy effect of mojabanchromanol isolated from Sargassum horneri in bone marrow-derived cultured mast cells

Abstract

This study was conducted to isolate and identify an active compound possessing anti-allergic property from the Jeju Island inhabiting Sargassum horneri (S. horneri). The ethanol extract of S. horneri (SHE) exhibited an anti-allergic effect via the reduction of β-hexosaminidase release in immunoglobulin E (IgE)/bovine serum albumin (BSA)-stimulated bone marrow-derived cultured mast cells (BMCMCs). SHE was subjected to liquid phase fractionation obtaining the hexane fraction, and this was further purified via ODS column chromatography and prep-C18 HPLC. The isolated compound was identified as mojabanchromanol (MC) by 1H, 13C NMR, and LCMS analysis. Isolated MC showed a higher inhibitory effect on the β-hexosaminidase release than those of SHE without cytotoxicity. Moreover, MC inhibited the mRNA expression levels of allergic cytokines in BMCMCs. This is the first report to inform on the anti-allergic property of MC isolated from S. horneri. In conclusion, SHE, as well as MC, is applicable in the development of functional health food, cosmetics, and pharmaceutical products exhibiting anti-allergic effects.