Production of flavonoids in callus cultures of Sophora flavescens Aiton

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Title
Production of flavonoids in callus cultures of Sophora flavescens Aiton
Author(s)
Ji-Sun Park; Zuh Kyung Seong; Mi Sun Kim; Jang Ho Ha; Ki-Beom Moon; Hyo Jun Lee; Hyeong Kyu Lee; Jae Heung Jeon; S U Park; Hyun Soon Kim
Bibliographic Citation
Plants-Basel, vol. 9, pp. 688-688
Publication Year
2020
Abstract
Flavonoids, including maackiain (Maac) from Sophora flavescens Aiton roots, have many pharmacological properties, such as antitumor, antimicrobial, and antifungal activities. This research aimed to develop an in vitro plant and callus culture system for S. flavescens for the purpose of generating an alternative production system for enhancing Maac production, as Maac is usually present in very small amounts in S. flavescens’ roots. We arranged the optimal conditions of different tissues of S. flavescens and supplemented the medium with various plant growth regulators (PGRs). The highest induction and proliferation rates of callus was shown in combination treatments of all concentrations of thidiazuron (TDZ) and picloram. In addition, calli induced with leaf explants cultured on 2.0 mg/L picloram and 0.5 mg/L 6-benzyladenine (BA) in Murashige and Skoog (MS) medium had the highest accumulation of the active metabolite Maac. In vitro shoots were regenerated on medium containing combinations of TDZ and α-Naphthalene acetic acid (NAA). A reliable protocol for the mass production of secondary metabolites using a callus culture of S. flavescens was successfully established.
Keyword
Callus inductionHerbal resourcePlant growth regulatorsSecondary metaboliteSophora flavescens
ISSN
2223-7747
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/plants9060688
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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