Effect of triclosan exposure on developmental competence in parthenogenetic porcine embryo during preimplantation

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Title
Effect of triclosan exposure on developmental competence in parthenogenetic porcine embryo during preimplantation
Author(s)
Min Ju Kim; H J Park; Sanghoon Lee; Hyo-Gu Kang; Pil Soo Jeong; Soo Hyun Park; Young-Ho ParkJong Hee LeeKyung Seob LimSeung Hwan LeeBo Woong SimSun-Uk Kim; S K Cho; D B Koo; Bong-Seok Song
Bibliographic Citation
International Journal of Molecular Sciences, vol. 21, no. 16, pp. 5790-5790
Publication Year
2020
Abstract
Triclosan (TCS) is included in various healthcare products because of its antimicrobial activity; therefore, many humans are exposed to TCS daily. While detrimental effects of TCS exposure have been reported in various species and cell types, the effects of TCS exposure on early embryonic development are largely unknown. The aim of this study was to determine if TCS exerts toxic effects during early embryonic development using porcine parthenogenetic embryos in vitro. Porcine parthenogenetic embryos were cultured in in vitro culture medium with 50 or 100 μM TCS for 6 days. Developmental parameters including cleavage and blastocyst formation rates, developmental kinetics, and the number of blastomeres were assessed. To determine the toxic effects of TCS, apoptosis, oxidative stress, and mitochondrial dysfunction were assessed. TCS exposure resulted in a significant decrease in 2-cell rate and blastocyst formation rate, as well as number of blastomeres, but not in the cleavage rate. TCS also increased the number of apoptotic blastomeres and the production of reactive oxygen species. Finally, TCS treatment resulted in a diffuse distribution of mitochondria and decreased the mitochondrial membrane potential. Our results showed that TCS exposure impaired porcine early embryonic development by inducing DNA damage, oxidative stress, and mitochondrial dysfunction.
Keyword
triclosanparthenogenetic embryodevelopmental competenceoxidative stressmitochondria dysfunction
ISSN
1422-0067
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/ijms21165790
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > National Primate Research Center > 1. Journal Articles
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