Defatted Chlorella biomass as a renewable carbon source for polyhydroxyalkanoates and carotenoids co-production

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Title
Defatted Chlorella biomass as a renewable carbon source for polyhydroxyalkanoates and carotenoids co-production
Author(s)
C Khomlaem; H Aloui; A R Deshmukh; Jin-Ho YunHee-Sik Kim; S C Napathorn; B S Kim
Bibliographic Citation
Algal Research-Biomass Biofuels and Bioproducts, vol. 51, pp. 102068-102068
Publication Year
2020
Abstract
Defatted Chlorella biomass was investigated as an unconventional and promising carbon source for the co-production of polyhydroxyalkanoates and cartenoids via batch cultivation of Paracoccus sp. LL1. 10% (w/v) of algal biomass was subjected to acid hydrolysis using different concentrations (0.1, 0.2, 0.3, and 0.5 N) of either sulfuric or hydrochloric acid to yield fermentable sugars. Regardless of the acid type, reducing sugar content significantly increased with an increasing acid concentration, reaching a maximum yield of 52 and 46 g/L respectively for hydrochloric and sulfuric acid catalyzed hydrolysis at the highest acid concentrations (p < 0.05). Shake flask fermentation process showed the ability of Paracoccus sp. LL1 to convert fermentable sugars from 0.3 N hydrochloric acid catalyzed hydrolysis into polyhydroxyalkanoates and carotenoids with a maximum product concentration of 1.48 g/L (i.e., 37.4% of dry cell weight) and 6.08 mg/L, respectively. Moreover, 144 and 92% increases in polyhydroxyalkanoates and carotenoids production (i.e., 3.62 g/L polyhydroxyalkanoates and 11.7 mg/L carotenoids) was recorded upon upscaling the biotransformation of defatted Chlorella biomass hydrolysate in 5 L fermenter by Paracoccus sp. LL1. Notably, these results corresponded to 92 and 54% greater production of polyhydroxyalkanoates and carotenoids than those observed in fermenter operated under identical conditions with glucose as a carbon source. Subsequent analyses of gas chromatography, Fourier transform infrared spectroscopy, and 1H nuclear magnetic resonance spectroscopy further identified the accumulated polyhydroxyalkanoate as a copolymer of 3-hydroxybutyrate and 3-hydroxyvalerate with a 3-hydroxyvalerate fraction of 6.22 mol%.
Keyword
Defatted Chlorella biomassParacoccus sp. LL1Acid hydrolysatePolyhydroxyalkanoatesCarotenoids
ISSN
2211-9264
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.algal.2020.102068
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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