The Gata1low murine megakaryocyte-erythroid progenitor cells expand robustly and alter differentiation potential

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The Gata1low murine megakaryocyte-erythroid progenitor cells expand robustly and alter differentiation potential
Eunju Shin; Jong Gwan Jeong; Hyunmin Chung; Haiyoung Jung; C Park; Suk Ran YoonTae-Don Kim; S J Lee; Inpyo Choi; Ji Yoon Noh
Bibliographic Citation
Biochemical and Biophysical Research Communications, vol. 528, pp. 46-53
Publication Year
GATA1 is a master transcription factor of megakaryopoiesis and erythropoiesis, and loss-of-function mutation can induce accumulation of megakaryocyte-erythroid progenitors (MEPs) in mice and humans. Accordingly, the murine MEP cell line (termed G1ME2 cells) encoding doxycycline (dox)-inducible anti-Gata1 shRNA on Hprt locus has been developed. The cells were CD41+CD71+KIT+, expand under dox, stem cell factor, and thrombopoietin (TPO), and terminally differentiate into erythroid cells or megakaryocytes upon removal of dox. Surprisingly, in this study, these Gata1low murine MEPs displayed accelerated growth from around 90-100 days after cell culture, impeded megakaryocytic potential, and maintained erythropoiesis. We specified them as late G1ME2 cells and discovered that increased CD41-KIT+ population during long-term culture was the main reason for the delayed megakaryopoiesis. The CD41 expression level was partially de-repressed by PI3K/AKT inhibitors, suggesting that TPO-mediated cell survival signaling pathway might have impacted on CD41 in the late G1ME2 cells. Nevertheless, among the late cells, the CD41+KIT+ cells could still generate megakaryocytes on dox withdrawal. Taken together, G1ME2 cells could provide a good model to study molecular mechanism of hematopoiesis because of their ability to expand excessively without artificial immortalization.
MegakaryopoiesisMegakaryocyte-erythroid progenitorCD41GATA1Cell proliferation
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Aging Convergence Research Center > 1. Journal Articles
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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