Overexpression of the golden SNP-carrying Orange gene enhances carotenoid accumulation and heat stress tolerance in sweetpotato plants

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Title
Overexpression of the golden SNP-carrying Orange gene enhances carotenoid accumulation and heat stress tolerance in sweetpotato plants
Author(s)
So-Eun Kim; Chan-Ju Lee; Sulu Park; Ye-Hoon Lim; W S Park; H J Kim; M J Ahn; Sang Soo KwakHo Soo Kim
Bibliographic Citation
Antioxidants, vol. 10, no. 1, pp. 51-51
Publication Year
2021
Abstract
Carotenoids function as photosynthetic accessory pigments, antioxidants, and vitamin A precursors. We recently showed that transgenic sweetpotato calli overexpressing the mutant sweetpotato (Ipomoea batatas [L.] Lam) Orange gene (IbOr-R96H), which carries a single nucleotide polymorphism responsible for Arg to His substitution at amino acid position 96, exhibited dramatically higher carotenoid content and abiotic stress tolerance than calli overexpressing the wild-type IbOr gene (IbOr-WT). In this study, we generated transgenic sweetpotato plants overexpressing IbOr-R96H under the control of the cauliflower mosaic virus (CaMV) 35S promoter via Agrobacterium-mediated transformation. The total carotenoid contents of IbOr-R96H storage roots (light-orange flesh) and IbOr-WT storage roots (light-yellow flesh) were 5.4?19.6 and 3.2-fold higher, respectively, than those of non-transgenic (NT) storage roots (white flesh). The β-carotene content of IbOr-R96H storage roots was up to 186.2-fold higher than that of NT storage roots. In addition, IbOr-R96H plants showed greater tolerance to heat stress (47 °C) than NT and IbOr-WT plants, possibly because of higher DPPH radical scavenging activity and ABA contents. These results indicate that IbOr-R96H is a promising strategy for developing new sweetpotato cultivars with improved carotenoid contents and heat stress tolerance.
Keyword
SweetpotatoCarotenoidOrangeIbOrIbOr-R96H
ISSN
2076-3921
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/antiox10010051
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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