Antagonistic control of genetic circuit performance for rapid analysis of targeted enzyme activity in living cells = 유전자회로의 길항적 제어를 이용한 세포 내 표적 효소 활성의 신속한 분석

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Title
Antagonistic control of genetic circuit performance for rapid analysis of targeted enzyme activity in living cells = 유전자회로의 길항적 제어를 이용한 세포 내 표적 효소 활성의 신속한 분석
Author(s)
Kil Koang KwonHaseong Kim; Soo Jin Yeom; Eugene Rha; Jinju Lee; Hyewon LeeDae Hee LeeSeung Goo Lee
Bibliographic Citation
Frontiers in Molecular Biosciences, vol. 7, pp. 599878-599878
Publication Year
2021
Abstract
Genetic circuits have been developed for quantitative measurement of enzyme activity, metabolic engineering of strain development, and dynamic regulation of microbial cells. A genetic circuit consists of several bio-elements, including enzymes and regulatory cassettes, that can generate the desired output signal, which is then used as a precise criterion for enzyme screening and engineering. Antagonists and inhibitors are small molecules with inhibitory effects on regulators and enzymes, respectively. In this study, an antagonist and an inhibitor were applied to a genetic circuit for a dynamic detection range. We developed a genetic circuit relying on regulators and enzymes, allowing for straightforward control of its output signal without additional genetic modification. We used para-nitrophenol and alanine as an antagonist of DmpR and inhibitor of tyrosine phenol-lyase, respectively. We show that the antagonist resets the detection range of the genetic circuit similarly to a resistor in an electrical logic circuit. These biological resistors in genetic circuits can be used as a rapid and precise controller of variable outputs with minimal circuit configuration.
Keyword
InhibitorAntagonistGenetic circuitPhenolic compoundFlow cytometryResistor
ISSN
2296-889X
Publisher
Frontiers Media Sa
DOI
http://dx.doi.org/10.3389/fmolb.2020.599878
Type
Article
Appears in Collections:
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
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