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- Antagonistic control of genetic circuit performance for rapid analysis of targeted enzyme activity in living cells = 유전자회로의 길항적 제어를 이용한 세포 내 표적 효소 활성의 신속한 분석
- Kil Koang Kwon; Haseong Kim; Soo Jin Yeom; Eugene Rha; Jinju Lee; Hyewon Lee; Dae Hee Lee; Seung Goo Lee
- Bibliographic Citation
- Frontiers in Molecular Biosciences, vol. 7, pp. 599878-599878
- Publication Year
- Genetic circuits have been developed for quantitative measurement of enzyme activity, metabolic engineering of strain development, and dynamic regulation of microbial cells. A genetic circuit consists of several bio-elements, including enzymes and regulatory cassettes, that can generate the desired output signal, which is then used as a precise criterion for enzyme screening and engineering. Antagonists and inhibitors are small molecules with inhibitory effects on regulators and enzymes, respectively. In this study, an antagonist and an inhibitor were applied to a genetic circuit for a dynamic detection range. We developed a genetic circuit relying on regulators and enzymes, allowing for straightforward control of its output signal without additional genetic modification. We used para-nitrophenol and alanine as an antagonist of DmpR and inhibitor of tyrosine phenol-lyase, respectively. We show that the antagonist resets the detection range of the genetic circuit similarly to a resistor in an electrical logic circuit. These biological resistors in genetic circuits can be used as a rapid and precise controller of variable outputs with minimal circuit configuration.
- InhibitorAntagonistGenetic circuitPhenolic compoundFlow cytometryResistor
- Frontiers Media Sa
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- Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > 1. Journal Articles
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