Evaluation of the acute toxicity of theoredoxin (TRX) transgenic soybean to Daphnia magna

Cited 0 time in scopus
Metadata Downloads
Title
Evaluation of the acute toxicity of theoredoxin (TRX) transgenic soybean to Daphnia magna
Author(s)
S D Oh; S K Min; J K Kim; Jung-Ho ParkChang-Gi Kim; S Y Park
Bibliographic Citation
Korean Journal of Agricultural Science, vol. 47, no. 4, pp. 791-802
Publication Year
2020
Abstract
Theoredoxin (TRX) transgenic soybeans were developed using the human Theoredoxin gene under the control of the β-conglycinin promoter with a selection marker, the phosphinothricin acetyltransferase (PAT) gene. This study was done to assess the acute toxicity of a genetically modified (GM) soybean using the fresh water planktonic crustacean Daphnia magna. The acute toxicity effect of the TRX soybean and non-GM soybean (Gwangan) on D. magna was investigated at different concentrations (0, 156, 313, 625, 1,250, 2,500, and 5,000 mg·L-1). The TRX soybean used for the test was confirmed to express the TRX/PAT genes by PCR and enzyme-linked immunosorbent assay (ELISA). D. magna feeding tests showed no significant differences in the cumulative immobility or an abnormal response with either the TRX soybean or non-GM soybean. The feeding study showed a similar abnormal response and cumulative immobility of the D. magna between the TRX soybean and Gwangan treatments. Additionally, the 48 h-EC50 values for the TRX and Gwangan soybeans were 755.6 and 778 mg·L-1, respectively. The soybean NOEC (no observed effect concentration) value for D. magna was suggested to be 156 mg·L-1. These results suggest that there is no significant difference in toxicity to Daphnia magna between the TRX soybean and its non-GM counterpart.
Keyword
Daphnia magnaEnvironment riskTheoredoxin soybean
ISSN
2466-2402
DOI
http://dx.doi.org/10.7744/kjoas.20200066
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Bio-Evaluation Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.