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- Title
- Evaluation of the acute toxicity of theoredoxin (TRX) transgenic soybean to Daphnia magna
- Author(s)
- S D Oh; S K Min; J K Kim; Jung-Ho Park; Chang-Gi Kim; S Y Park
- Bibliographic Citation
- Korean Journal of Agricultural Science, vol. 47, no. 4, pp. 791-802
- Publication Year
- 2020
- Abstract
- Theoredoxin (TRX) transgenic soybeans were developed using the human Theoredoxin gene under the control of the β-conglycinin promoter with a selection marker, the phosphinothricin acetyltransferase (PAT) gene. This study was done to assess the acute toxicity of a genetically modified (GM) soybean using the fresh water planktonic crustacean Daphnia magna. The acute toxicity effect of the TRX soybean and non-GM soybean (Gwangan) on D. magna was investigated at different concentrations (0, 156, 313, 625, 1,250, 2,500, and 5,000 mg·L-1). The TRX soybean used for the test was confirmed to express the TRX/PAT genes by PCR and enzyme-linked immunosorbent assay (ELISA). D. magna feeding tests showed no significant differences in the cumulative immobility or an abnormal response with either the TRX soybean or non-GM soybean. The feeding study showed a similar abnormal response and cumulative immobility of the D. magna between the TRX soybean and Gwangan treatments. Additionally, the 48 h-EC50 values for the TRX and Gwangan soybeans were 755.6 and 778 mg·L-1, respectively. The soybean NOEC (no observed effect concentration) value for D. magna was suggested to be 156 mg·L-1. These results suggest that there is no significant difference in toxicity to Daphnia magna between the TRX soybean and its non-GM counterpart.
- Keyword
- Daphnia magnaEnvironment riskTheoredoxin soybean
- ISSN
- 2466-2402
- Publisher
- Korea Soc-Assoc-Inst
- DOI
- http://dx.doi.org/10.7744/kjoas.20200066
- Type
- Article
- Appears in Collections:
- Division of Bio Technology Innovation > Bio-Evaluation Center > 1. Journal Articles
- Files in This Item:
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