A pipecolic acid-rich branched cyclic depsipeptide ulleungamide C from a Streptomyces species induces G0/G1 cell cycle arrest in promyelocytic leukemia cells

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Title
A pipecolic acid-rich branched cyclic depsipeptide ulleungamide C from a Streptomyces species induces G0/G1 cell cycle arrest in promyelocytic leukemia cells
Author(s)
Sangkeun Son; Mina Jang; Byeongsan Lee; Jun-Pil JangYoung-Soo HongBo Yeon KimSung-Kyun KoJae-Hyuk JangJong Seog Ahn
Bibliographic Citation
Journal of Antibiotics, vol. 74, no. 3, pp. 181-189
Publication Year
2021
Abstract
In this study, screening by LC-MS and cytotoxicity-guided isolation led to the identification of ulleungamide C (1), a previously unknown pipecolic acid-rich branched cyclic depsipeptide, from a soil actinobacterium Streptomyces sp. KCB13F003. The structure of 1 was determined by interpretation of spectroscopic and spectrometric data from 1D and 2D NMR and HRESIMS experiments. Antiproliferative assays using mammalian cancerous cells revealed that 1 inhibits the proliferation of HL-60 human promyelocytic leukemia cells. Cell cycle analysis showed an increased accumulation of cells in the G0/G1 phase after treatment with 1. Results of immunoblotting assays revealed that 1 reduced the expression levels of cyclin-dependent kinase 4 (CDK4), CDK6, retinoblastoma protein (Rb), and phosphorylated Rb, whereas it induced cyclin-dependent kinase inhibitor 1B (p27/Kip1) expression.
ISSN
0021-8820
Publisher
Springer-Nature Pub Group
DOI
http://dx.doi.org/10.1038/s41429-020-00385-z
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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