Phage display screening of bovine antibodies to foot-and-mouth disease virus and their application in a competitive ELISA for serodiagnosis

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Title
Phage display screening of bovine antibodies to foot-and-mouth disease virus and their application in a competitive ELISA for serodiagnosis
Author(s)
Sukyo Jeong; Hyun Joo Ahn; Kyung-Jin Min; J W Byun; H M Pyo; M Y Park; B KKu; J Nah; S Ryoo; S H Wee; Sang Jick Kim
Bibliographic Citation
International Journal of Molecular Sciences, vol. 22, no. 9, pp. 4328-4328
Publication Year
2021
Abstract
For serodiagnosis of foot-and-mouth disease virus (FMDV), monoclonal antibody (MAb)-based competitive ELISA (cELISA) is commonly used since it allows simple and reproducible detection of antibody response to FMDV. However, the use of mouse-origin MAb as a detection reagent is questionable, as antibody responses to FMDV in mice may differ in epitope structure and preference from those in natural hosts such as cattle and pigs. To take advantage of natural host-derived antibodies, a phage-displayed scFv library was constructed from FMDV-immune cattle and subjected to two separate pannings against inactivated FMDV type O and A. Subsequent ELISA screening revealed high-affinity scFv antibodies specific to a serotype (O or A) as well as those with pan-serotype specificity. When BvO17, an scFv antibody specific to FMDV type O, was tested as a detection reagent in cELISA, it successfully detected FMDV type O antibodies for both serum samples from vaccinated cattle and virus-challenged pigs with even higher sensitivity than a mouse MAb-based commercial FMDV type O antibody detection kit. These results demonstrate the feasibility of using natural host-derived antibodies such as bovine scFv instead of mouse MAb in cELISA for serological detection of antibody response to FMDV in the susceptible animals.
Keyword
Foot-and-mouth disease virusType OType APhage displayantibodyCompetitive ELISA
ISSN
1661-6596
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/ijms22094328
Type
Article
Appears in Collections:
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
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