Functional characterization of the mazEF toxin-antitoxin system in the pathogenic bacterium Agrobacterium tumefaciens

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dc.contributor.authorWonho Choi-
dc.contributor.authorY Yamaguchi-
dc.contributor.authorJi-Young Park-
dc.contributor.authorSang-Hyun Park-
dc.contributor.authorHyeok Won Lee-
dc.contributor.authorB K Lim-
dc.contributor.authorM Otto-
dc.contributor.authorM Inouye-
dc.contributor.authorM H Yoon-
dc.contributor.authorJung-Ho Park-
dc.date.accessioned2021-06-03T03:31:01Z-
dc.date.available2021-06-03T03:31:01Z-
dc.date.issued2021-
dc.identifier.issn20762607-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/24373-
dc.description.abstractAgrobacterium tumefaciens is a pathogen of various plants which transfers its own DNA (T-DNA) to the host plants. It is used for producing genetically modified plants with this ability. To control T-DNA transfer to the right place, toxin-antitoxin (TA) systems of A. tumefaciens were used to control the target site of transfer without any unintentional targeting. Here, we describe a toxin-antitoxin system, Atu0939 (mazE-at) and Atu0940 (mazF-at), in the chromosome of Agrobacterium tumefaciens. The toxin in the TA system has 33.3% identity and 45.5% similarity with MazF in Escherichia coli. The expression of MazF-at caused cell growth inhibition, while cells with MazF-at co-expressed with MazE-at grew normally. In vivo and in vitro assays revealed that MazF-at inhibited protein synthesis by decreasing the cellular mRNA stability. Moreover, the catalytic residue of MazF-at was determined to be the 24th glutamic acid using site-directed mutagenesis. From the results, we concluded that MazF-at is a type II toxin-antitoxin system and a ribosome-independent endoribonuclease. Here, we characterized a TA system in A. tumefaciens whose understanding might help to find its physiological function and to develop further applications.-
dc.publisherMDPI-
dc.titleFunctional characterization of the mazEF toxin-antitoxin system in the pathogenic bacterium Agrobacterium tumefaciens-
dc.title.alternativeFunctional characterization of the mazEF toxin-antitoxin system in the pathogenic bacterium Agrobacterium tumefaciens-
dc.typeArticle-
dc.citation.titleMicroorganisms-
dc.citation.number5-
dc.citation.endPage1107-
dc.citation.startPage1107-
dc.citation.volume9-
dc.contributor.affiliatedAuthorWonho Choi-
dc.contributor.affiliatedAuthorJi-Young Park-
dc.contributor.affiliatedAuthorSang-Hyun Park-
dc.contributor.affiliatedAuthorHyeok Won Lee-
dc.contributor.affiliatedAuthorJung-Ho Park-
dc.contributor.alternativeName최원호-
dc.contributor.alternativeNameYamaguchi-
dc.contributor.alternativeName박지영-
dc.contributor.alternativeName박상현-
dc.contributor.alternativeName이혁원-
dc.contributor.alternativeName임병관-
dc.contributor.alternativeNameOtto-
dc.contributor.alternativeNameInouye-
dc.contributor.alternativeName윤민호-
dc.contributor.alternativeName박정호-
dc.identifier.bibliographicCitationMicroorganisms, vol. 9, no. 5, pp. 1107-1107-
dc.identifier.doi10.3390/microorganisms9051107-
dc.subject.keywordAgrobacterium tumefaciens-
dc.subject.keywordTA system-
dc.subject.keywordmazF-
dc.subject.keywordMazE-
dc.subject.keywordmRNA endonuclease-
dc.subject.localAgrobacterium tumefaciens-
dc.description.journalClassY-
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Bio-Evaluation Center > 1. Journal Articles
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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