DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wonho Choi | - |
dc.contributor.author | Y Yamaguchi | - |
dc.contributor.author | Ji-Young Park | - |
dc.contributor.author | Sang-Hyun Park | - |
dc.contributor.author | Hyeok Won Lee | - |
dc.contributor.author | B K Lim | - |
dc.contributor.author | M Otto | - |
dc.contributor.author | M Inouye | - |
dc.contributor.author | M H Yoon | - |
dc.contributor.author | Jung-Ho Park | - |
dc.date.accessioned | 2021-06-03T03:31:01Z | - |
dc.date.available | 2021-06-03T03:31:01Z | - |
dc.date.issued | 2021 | - |
dc.identifier.issn | 2076-2607 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/24373 | - |
dc.description.abstract | Agrobacterium tumefaciens is a pathogen of various plants which transfers its own DNA (T-DNA) to the host plants. It is used for producing genetically modified plants with this ability. To control T-DNA transfer to the right place, toxin-antitoxin (TA) systems of A. tumefaciens were used to control the target site of transfer without any unintentional targeting. Here, we describe a toxin-antitoxin system, Atu0939 (mazE-at) and Atu0940 (mazF-at), in the chromosome of Agrobacterium tumefaciens. The toxin in the TA system has 33.3% identity and 45.5% similarity with MazF in Escherichia coli. The expression of MazF-at caused cell growth inhibition, while cells with MazF-at co-expressed with MazE-at grew normally. In vivo and in vitro assays revealed that MazF-at inhibited protein synthesis by decreasing the cellular mRNA stability. Moreover, the catalytic residue of MazF-at was determined to be the 24th glutamic acid using site-directed mutagenesis. From the results, we concluded that MazF-at is a type II toxin-antitoxin system and a ribosome-independent endoribonuclease. Here, we characterized a TA system in A. tumefaciens whose understanding might help to find its physiological function and to develop further applications. | - |
dc.publisher | MDPI | - |
dc.title | Functional characterization of the mazEF toxin-antitoxin system in the pathogenic bacterium Agrobacterium tumefaciens | - |
dc.title.alternative | Functional characterization of the mazEF toxin-antitoxin system in the pathogenic bacterium Agrobacterium tumefaciens | - |
dc.type | Article | - |
dc.citation.title | Microorganisms | - |
dc.citation.number | 5 | - |
dc.citation.endPage | 1107 | - |
dc.citation.startPage | 1107 | - |
dc.citation.volume | 9 | - |
dc.contributor.affiliatedAuthor | Wonho Choi | - |
dc.contributor.affiliatedAuthor | Ji-Young Park | - |
dc.contributor.affiliatedAuthor | Sang-Hyun Park | - |
dc.contributor.affiliatedAuthor | Hyeok Won Lee | - |
dc.contributor.affiliatedAuthor | Jung-Ho Park | - |
dc.contributor.alternativeName | 최원호 | - |
dc.contributor.alternativeName | Yamaguchi | - |
dc.contributor.alternativeName | 박지영 | - |
dc.contributor.alternativeName | 박상현 | - |
dc.contributor.alternativeName | 이혁원 | - |
dc.contributor.alternativeName | 임병관 | - |
dc.contributor.alternativeName | Otto | - |
dc.contributor.alternativeName | Inouye | - |
dc.contributor.alternativeName | 윤민호 | - |
dc.contributor.alternativeName | 박정호 | - |
dc.identifier.bibliographicCitation | Microorganisms, vol. 9, no. 5, pp. 1107-1107 | - |
dc.identifier.doi | 10.3390/microorganisms9051107 | - |
dc.subject.keyword | Agrobacterium tumefaciens | - |
dc.subject.keyword | TA system | - |
dc.subject.keyword | mazF | - |
dc.subject.keyword | MazE | - |
dc.subject.keyword | mRNA endonuclease | - |
dc.subject.local | agrobacterium tumefaciens | - |
dc.subject.local | Agrobacterium tumefaciens | - |
dc.subject.local | TA systems | - |
dc.subject.local | TA system | - |
dc.subject.local | mazF | - |
dc.subject.local | MazF | - |
dc.subject.local | MazE | - |
dc.subject.local | mRNA endonuclease | - |
dc.description.journalClass | Y | - |
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