The groESL ISR sequence-based species-specific identification of GRAS and non-GRAS Lactiplantibacillus as an alternative to 16S rRNA sequencing

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Title
The groESL ISR sequence-based species-specific identification of GRAS and non-GRAS Lactiplantibacillus as an alternative to 16S rRNA sequencing
Author(s)
Jong Min Lee; So Hee Park; C Z Jin; Min-Kyoung Kang; Dong Jin ParkChang-Jin Kim
Bibliographic Citation
LWT-Food Science and Technology, vol. 147, pp. 111504-111504
Publication Year
2021
Abstract
The untranscribed DNA sequences of the intergenic spacer regions (ISR) in the groESL were analyzed to resolve the ambiguity within phylogenetically close GRAS and non-GRAS species. The sequencing results of amplified polymerase chain reaction (PCR) products using groESL-ISR-specific primers accurately distinguished L. plantarum and L. pentosus, which were not distinguished by 16 S rRNA sequences. Furthermore, the 20 selected major probiotics species were divided into several groups according to the length (22?91 bp) and homology (65?99%) of their ISR sequences, and this discrimination was consistent with core-genome-based differentiation. Therefore, ISR sequence-based species identification showed more accurate results than 16 S rRNA sequence-based identification and represented genome-based speciation.
Keyword
IdentificationLactiplantibacillusLactobacillusIntergenic spacer regiongroESL
ISSN
0023-6438
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.lwt.2021.111504
Type
Article
Appears in Collections:
Division of Biomaterials Research > Industrial Bio-materials Research Center > 1. Journal Articles
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