Anti-inflammatory and antioxidant effects of Soroseris hirsuta extract by regulating iNOS/NF-κB and NRF2/HO-1 pathways in murine macrophage RAW 264.7 cells

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Title
Anti-inflammatory and antioxidant effects of Soroseris hirsuta extract by regulating iNOS/NF-κB and NRF2/HO-1 pathways in murine macrophage RAW 264.7 cells
Author(s)
W J Lee; W Y Li; Sang Woo Lee; S K Jung
Bibliographic Citation
Applied Sciences-Basel, vol. 11, no. 10, pp. 4711-4711
Publication Year
2021
Abstract
Until now, the physiological effects of Soroseris hirsuta were primarily unknown. Here we have evaluated the anti-inflammatory and antioxidant effects of Soroseris hirsuta extract (SHE) on lipopolysaccharide (LPS)-activated murine macrophages RAW 264.7 cells. SHE inhibited nitric oxide expression and inducible nitric oxide synthase expression in RAW 264.7 cells treated with LPS. Moreover, SHE suppressed LPS-induced phosphorylation of IκB kinase, inhibitor of kappa B, p65, p38, and c-JUN N-terminal kinase. Western blot and immunofluorescence analyses showed that SHE suppressed p65 nuclear translocation induced by LPS. Furthermore, SHE inhibited the reactive oxygen species in LPS-treated RAW 264.7 cells. SHE significantly increased heme oxygenase-1 expression and the nuclear translocation of nuclear factor erythroid 2-related factor 2. SHE suppressed LPS-induced interleukin-1β mRNA expression in RAW 264.7 cells. Thus, SHE is a promising nutraceutical as it displays anti-inflammatory and antioxidant properties.
Keyword
Soroseris hirsutaAnti-inflammationAntioxidantNitric oxideHeme oxygenase-1
ISSN
2076-3417
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/app11104711
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > International Biological Material Research Center > 1. Journal Articles
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