DC Field | Value | Language |
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dc.contributor.author | Hyun Ji Park | - |
dc.contributor.author | Hae-Myeong Jung | - |
dc.contributor.author | Areum Lee | - |
dc.contributor.author | Seung Hee Jo | - |
dc.contributor.author | Hyo Jun Lee | - |
dc.contributor.author | Hyun-Soon Kim | - |
dc.contributor.author | C K Jung | - |
dc.contributor.author | Sung Ran Min | - |
dc.contributor.author | Hye Sun Cho | - |
dc.date.accessioned | 2021-08-25T15:30:21Z | - |
dc.date.available | 2021-08-25T15:30:21Z | - |
dc.date.issued | 2021 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/24671 | - |
dc.description.abstract | OsFKBP20-1b, a plant-specific cyclophilin protein, has been implicated to regulate pre-mRNA splicing under stress conditions in rice. Here, we demonstrated that OsFKBP20-1b is SUMOylated in a reconstituted SUMOylation system in E.coli and in planta, and that the SUMOylation-coupled regulation was associated with enhanced protein stability using a less SUMOylated OsFKBP20-1b mutant (5KR_OsFKBP20-1b). Furthermore, OsFKBP20-1b directly interacted with OsSUMO1 and OsSUMO2 in the nucleus and cytoplasm, whereas the less SUMOylated 5KR_OsFKBP20-1b mutant had an impaired interaction with OsSUMO1 and 2 in the cytoplasm but not in the nucleus. Under heat stress, the abundance of an OsFKBP20-1b-GFP fusion protein was substantially increased in the nuclear speckles and cytoplasmic foci, whereas the heat-responsiveness was remarkably diminished in the presence of the less SUMOylated 5KR_OsFKBP20-1b-GFP mutant. The accumulation of endogenous SUMOylated OsFKBP20-1b was enhanced by heat stress in planta. Moreover, 5KR_OsFKBP20-1b was not sufficiently associated with the U snRNAs in the nucleus as a spliceosome component. A protoplast transfection assay indicated that the low SUMOylation level of 5KR_OsFKBP20-1b led to inaccurate alternative splicing and transcription under heat stress. Thus, our results suggest that OsFKBP20-1b is post-translationally regulated by SUMOylation, and the modification is crucial for proper RNA processing in response to heat stress in rice. | - |
dc.publisher | MDPI | - |
dc.title | SUMO modification of OsFKBP20-1b is integral to proper pre-mRNA splicing upon heat stress in rice = 벼 OsFKBP20-1b 단백질의 스모화 변형을 통한 고온스트레스 pre-mRNA 스플라이싱 조절 | - |
dc.title.alternative | SUMO modification of OsFKBP20-1b is integral to proper pre-mRNA splicing upon heat stress in rice | - |
dc.type | Article | - |
dc.citation.title | International Journal of Molecular Sciences | - |
dc.citation.number | 16 | - |
dc.citation.endPage | 9049 | - |
dc.citation.startPage | 9049 | - |
dc.citation.volume | 22 | - |
dc.contributor.affiliatedAuthor | Hyun Ji Park | - |
dc.contributor.affiliatedAuthor | Hae-Myeong Jung | - |
dc.contributor.affiliatedAuthor | Areum Lee | - |
dc.contributor.affiliatedAuthor | Seung Hee Jo | - |
dc.contributor.affiliatedAuthor | Hyo Jun Lee | - |
dc.contributor.affiliatedAuthor | Hyun-Soon Kim | - |
dc.contributor.affiliatedAuthor | Sung Ran Min | - |
dc.contributor.affiliatedAuthor | Hye Sun Cho | - |
dc.contributor.alternativeName | 박현지 | - |
dc.contributor.alternativeName | 정해명 | - |
dc.contributor.alternativeName | 이아름 | - |
dc.contributor.alternativeName | 조승희 | - |
dc.contributor.alternativeName | 이효준 | - |
dc.contributor.alternativeName | 김현순 | - |
dc.contributor.alternativeName | 정춘균 | - |
dc.contributor.alternativeName | 민성란 | - |
dc.contributor.alternativeName | 조혜선 | - |
dc.identifier.bibliographicCitation | International Journal of Molecular Sciences, vol. 22, no. 16, pp. 9049-9049 | - |
dc.identifier.doi | 10.3390/ijms22169049 | - |
dc.subject.keyword | SUMOylation | - |
dc.subject.keyword | OsFKBP20-1b | - |
dc.subject.keyword | Splicing factor | - |
dc.subject.keyword | Heat stress | - |
dc.subject.keyword | pre-mRNA splicing | - |
dc.subject.local | SUMOylation | - |
dc.subject.local | OsFKBP20-1b | - |
dc.subject.local | Splicing factor | - |
dc.subject.local | heat stress | - |
dc.subject.local | Heat stress | - |
dc.subject.local | Pre-mRNA splicing | - |
dc.subject.local | pre-mRNA splicing | - |
dc.description.journalClass | Y | - |
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