SUMO modification of OsFKBP20-1b is integral to proper pre-mRNA splicing upon heat stress in rice = 벼 OsFKBP20-1b 단백질의 스모화 변형을 통한 고온스트레스 pre-mRNA 스플라이싱 조절

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dc.contributor.authorHyun Ji Park-
dc.contributor.authorHae-Myeong Jung-
dc.contributor.authorAreum Lee-
dc.contributor.authorSeung Hee Jo-
dc.contributor.authorHyo Jun Lee-
dc.contributor.authorHyun-Soon Kim-
dc.contributor.authorC K Jung-
dc.contributor.authorSung Ran Min-
dc.contributor.authorHye Sun Cho-
dc.date.accessioned2021-08-25T15:30:21Z-
dc.date.available2021-08-25T15:30:21Z-
dc.date.issued2021-
dc.identifier.issn16616596-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/24671-
dc.description.abstractOsFKBP20-1b, a plant-specific cyclophilin protein, has been implicated to regulate pre-mRNA splicing under stress conditions in rice. Here, we demonstrated that OsFKBP20-1b is SUMOylated in a reconstituted SUMOylation system in E.coli and in planta, and that the SUMOylation-coupled regulation was associated with enhanced protein stability using a less SUMOylated OsFKBP20-1b mutant (5KR_OsFKBP20-1b). Furthermore, OsFKBP20-1b directly interacted with OsSUMO1 and OsSUMO2 in the nucleus and cytoplasm, whereas the less SUMOylated 5KR_OsFKBP20-1b mutant had an impaired interaction with OsSUMO1 and 2 in the cytoplasm but not in the nucleus. Under heat stress, the abundance of an OsFKBP20-1b-GFP fusion protein was substantially increased in the nuclear speckles and cytoplasmic foci, whereas the heat-responsiveness was remarkably diminished in the presence of the less SUMOylated 5KR_OsFKBP20-1b-GFP mutant. The accumulation of endogenous SUMOylated OsFKBP20-1b was enhanced by heat stress in planta. Moreover, 5KR_OsFKBP20-1b was not sufficiently associated with the U snRNAs in the nucleus as a spliceosome component. A protoplast transfection assay indicated that the low SUMOylation level of 5KR_OsFKBP20-1b led to inaccurate alternative splicing and transcription under heat stress. Thus, our results suggest that OsFKBP20-1b is post-translationally regulated by SUMOylation, and the modification is crucial for proper RNA processing in response to heat stress in rice.-
dc.publisherMDPI-
dc.titleSUMO modification of OsFKBP20-1b is integral to proper pre-mRNA splicing upon heat stress in rice = 벼 OsFKBP20-1b 단백질의 스모화 변형을 통한 고온스트레스 pre-mRNA 스플라이싱 조절-
dc.title.alternativeSUMO modification of OsFKBP20-1b is integral to proper pre-mRNA splicing upon heat stress in rice-
dc.typeArticle-
dc.citation.titleInternational Journal of Molecular Sciences-
dc.citation.number16-
dc.citation.endPage9049-
dc.citation.startPage9049-
dc.citation.volume22-
dc.contributor.affiliatedAuthorHyun Ji Park-
dc.contributor.affiliatedAuthorHae-Myeong Jung-
dc.contributor.affiliatedAuthorAreum Lee-
dc.contributor.affiliatedAuthorSeung Hee Jo-
dc.contributor.affiliatedAuthorHyo Jun Lee-
dc.contributor.affiliatedAuthorHyun-Soon Kim-
dc.contributor.affiliatedAuthorSung Ran Min-
dc.contributor.affiliatedAuthorHye Sun Cho-
dc.contributor.alternativeName박현지-
dc.contributor.alternativeName정해명-
dc.contributor.alternativeName이아름-
dc.contributor.alternativeName조승희-
dc.contributor.alternativeName이효준-
dc.contributor.alternativeName김현순-
dc.contributor.alternativeName정춘균-
dc.contributor.alternativeName민성란-
dc.contributor.alternativeName조혜선-
dc.identifier.bibliographicCitationInternational Journal of Molecular Sciences, vol. 22, no. 16, pp. 9049-9049-
dc.identifier.doi10.3390/ijms22169049-
dc.subject.keywordSUMOylation-
dc.subject.keywordOsFKBP20-1b-
dc.subject.keywordSplicing factor-
dc.subject.keywordHeat stress-
dc.subject.keywordpre-mRNA splicing-
dc.subject.localSUMOylation-
dc.subject.localOsFKBP20-1b-
dc.subject.localHeat stress-
dc.description.journalClassY-
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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