Human blood vessel organoids penetrate human cerebral organoids and form a vessel-like system

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Title
Human blood vessel organoids penetrate human cerebral organoids and form a vessel-like system
Author(s)
Yujin Ahn; Ju-hyun An; Hae Jun Yang; Dong Gil Lee; Jieun Kim; Hyebin Koh; Young-Ho ParkBong-Seok SongBo Woong Sim; H J Lee; Jong-Hee LeeSun-Uk Kim
Bibliographic Citation
Cells, vol. 10, no. 8, pp. 2036-2036
Publication Year
2021
Abstract
Vascularization of tissues, organoids and organ-on-chip models has been attempted using endothelial cells. However, the cultured endothelial cells lack the capacity to interact with other somatic cell types, which is distinct from developing vascular cells in vivo. Recently, it was demonstrated that blood vessel organoids (BVOs) recreate the structure and functions of developing human blood vessels. However, the tissue-specific adaptability of BVOs had not been assessed in somatic tissues. Herein, we investigated whether BVOs infiltrate human cerebral organoids and form a blood-brain barrier. As a result, vascular cells arising from BVOs penetrated the cerebral organoids and developed a vessel-like architecture composed of CD31+ endothelial tubes coated with SMA+ or PDGFR+ mural cells. Molecular markers of the blood-brain barrier were detected in the vascularized cerebral organoids. We revealed that BVOs can form neural-specific blood-vessel networks that can be maintained for over 50 days.
Keyword
VascularizationBlood vessel organoidCerebral organoidBlood-brain barrierVessel structureOrganoidsBlood vessel formationBrain organoid
ISSN
2073-4409
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/cells10082036
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > National Primate Research Center > 1. Journal Articles
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