Development of antibody against drug-resistant respiratory syncytial virus: rapid detection of mutant virus using split superfolder green fluorescent protein-antibody system
Cited 0 time in
- Development of antibody against drug-resistant respiratory syncytial virus: rapid detection of mutant virus using split superfolder green fluorescent protein-antibody system
- Hyeran Kim; Seul Gee Hwang; Kyeonghye Guk; Yoonji Bae; H Park; Eun Kyung Lim; Taejoon Kang; Juyeon Jung
- Bibliographic Citation
- Biosensors & Bioelectronics, vol. 194, pp. 113593-113593
- Publication Year
- Respiratory syncytial virus (RSV) infections are associated with severe bronchiolitis or pneumonia. Although palivizumab is used to prevent RSV infections, the occurrence of palivizumab-resistant RSV strains is increasing, and these strains pose a threat to public health. Herein, we report an antibody with affinity to the S275F RSV antigen, enabling the specific detection of palivizumab-resistant RSV strains. Experimental and simulation results confirmed the affinity of the antibody to the S275F RSV antigen. Furthermore, we developed a rapid S275F RSV antigen detection method using a split superfolder green fluorescent protein (ssGFP) that can interact with the antibody. In the presence of the mutant virus antigen, ssGFP emitted fluorescence within 1 min, allowing the rapid identification of S275F RSV. We anticipate that the developed antibody would be useful for the precise diagnosis of antiviral drug-resistant RSV strains and help treat patients with RSV infections.
- Antibody; Drug-resistance; S275F mutation; Respiratory syncytial virus; Green fluorescent protein
- Appears in Collections:
- Division of Biomaterials Research > Bionanotechnology Research Center > 1. Journal Articles
- Files in This Item:
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.