Anti-mullerian hormone negatively regulates osteoclast differentiation by suppressing the receptor activator of nuclear factor-κB ligand pathway

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Title
Anti-mullerian hormone negatively regulates osteoclast differentiation by suppressing the receptor activator of nuclear factor-κB ligand pathway
Author(s)
J H Kim; Yong Ryoul YangKi-Sun Kwon; N Kim
Bibliographic Citation
Journal of Bone Metabolism, vol. 28, no. 3, pp. 223-230
Publication Year
2021
Abstract
Background: Multiple members of the transforming growth factor-β (TGF-β) superfamily have well-established roles in bone homeostasis. Anti-Mullerian hormone (AMH) is a member of TGF-β superfamily of glycoproteins that is responsible for the regression of fetal Mullerian ducts and the transcription inhibition of gonadal steroidogenic enzymes. However, the involvement of AMH in bone remodeling is unknown. Therefore, we investigated whether AMH has an effect on bone cells as other TGF-β superfamily members do. Methods: To identify the roles of AMH in bone cells, we administered AMH during osteoblast and osteoclast differentiation, cultured the cells, and then stained the cultured cells with Alizarin red and tartrate-resistant acid phosphatase, respectively. We analyzed the expression of osteoblast- or osteoclast-related genes using real-time polymerase chain reaction and western blot. Results: AMH does not affect bone morphogenetic protein 2-mediated osteoblast differentiation but inhibits receptor activator of nuclear factor-κB (NF-κB) ligand-induced osteoclast differentiation. The inhibitory effect of AMH on osteoclast differentiation is mediated by IκB-NF-κB signaling. Conclusions: AMH negatively regulates osteoclast differentiation without affecting osteoblast differentiation.
Keyword
Anti-mullerian hormoneOsteoblastsOsteoclastsTGF-beta superfamily proteins
ISSN
2287-6375
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.11005/jbm.2021.28.3.223
Type
Article
Appears in Collections:
Division of Research on National Challenges > Aging Research Center > 1. Journal Articles
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