Four-week repeated intravenous dose toxicity of self-assembled-micelle inhibitory RNA-targeting amphiregulin in mice

Cited 1 time in scopus
Metadata Downloads
Title
Four-week repeated intravenous dose toxicity of self-assembled-micelle inhibitory RNA-targeting amphiregulin in mice
Author(s)
H Y Kim; T R Kim; S H Kim; I H Kim; J O Lim; J H Park; S Yun; In Chul Lee; H O Park; J C Kim
Bibliographic Citation
International Journal of Toxicology, vol. 40, no. 5, pp. 453-465
Publication Year
2021
Abstract
The present study investigated the potential subchronic toxicity of self-assembled-micelle inhibitory RNA-targeting amphiregulin (SAMiRNA-AREG) in mice. The test reagent was administered once-daily by intravenous injection for 4 weeks at 0, 100, 200, or 300 mg/kg/day doses. Additional recovery groups (vehicle control and high dose groups) were observed for a 2-week recovery period. During the test period, mortality, clinical signs, body weight, food consumption, ophthalmology, urinalysis, hematology, serum biochemistry, gross pathology, organ weight, and histopathology were examined. An increase in the percentages of basophil and large unstained cells was observed in the 200 and 300 mg/kg/day groups of both sexes. In addition, the absolute and relative weights of the spleen were higher in males given 300 mg/kg/day relative to the concurrent controls. However, these findings were considered of no toxicological significance because the changes were minimal, were not accompanied by other relevant results (eg, correlating microscopic changes), and were not observed at the end of the 2-week recovery period indicating recovery of the findings. Based on the results, SAMiRNA-AREG did not cause treatment-related adverse effects at dose levels of up to 300 mg/kg/day in mice after 4-week repeated intravenous doses. Under these conditions, the no-observed-adverse-effect level of the SAMiRNA-AREG was ≥300 mg/kg/day in both sexes and no target organs were identified.
Keyword
Self-assembled-micelle inhibitory RNA nanoparticleAmphiregulinRepeated dose toxicityNo-observed-adverse-effect levelTarget organ
ISSN
1091-5818
Publisher
Sage
DOI
http://dx.doi.org/10.1177/10915818211031241
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.