Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2

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Title
Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2
Author(s)
Hyunjung Cha; Hyeran Kim; Y Joung; Hyunju Kang; Jeong Moon; Hyowon Jang; S Park; Hyung-Jun KwonIn Chul Lee; S Kim; D Yong; Sun Woo Yoon; S G Park; Kyeonghye Guk; Eun Kyung Lim; H G Park; J Choo; Juyeon JungTaejoon Kang
Bibliographic Citation
Biosensors & Bioelectronics, vol. 202, pp. 114008-114008
Publication Year
2022
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected humans worldwide for over a year now. Although various tests have been developed for the detection of SARS-CoV-2, advanced sensing methods are required for the diagnosis, screening, and surveillance of coronavirus disease 2019 (COVID-19). Here, we report a surface-enhanced Raman scattering (SERS)-based immunoassay involving an antibody pair, SERS-active hollow Au nanoparticles (NPs), and magnetic beads for the detection of SARS-CoV-2. The selected antibody pair against the SARS-CoV-2 antigen, along with the magnetic beads, facilitates the accurate direct detection of the virus. The hollow Au NPs exhibit strong, reproducible SERS signals, allowing sensitive quantitative detection of SARS-CoV-2. This assay had detection limits of 2.56 fg/mL for the SARS-CoV-2 antigen and 3.4 plaque-forming units/mL for the SARS-CoV-2 lysates. Furthermore, it facilitated the identification of SARS-CoV-2 in human nasopharyngeal aspirates and diagnosis of COVID-19 within 30 min using a portable Raman device. Thus, this assay can be potentially used for the diagnosis and prevention of COVID-19.
Keyword
AntibodyCoronavirus disease 2019ImmunoassayNanoparticleSurface-enhanced Raman scatteringSevere acute respiratory syndrome coronavirus 2
ISSN
0956-5663
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.bios.2022.114008
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
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