Humibacter ginsenosidimutans sp. nov., with ginsenoside-converting activity isolated from activated sludge

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Title
Humibacter ginsenosidimutans sp. nov., with ginsenoside-converting activity isolated from activated sludge
Author(s)
G M Choi; Y W Lee; Hanna ChoeSo Yeon Kim; W T Im
Bibliographic Citation
International Journal of Systematic and Evolutionary Microbiology, vol. 72, no. 7, pp. 005442-005442
Publication Year
2022
Abstract
A novel Gram-stain-positive, thin rod-shaped, non-motile, aerobic and creamy-white pigmented bacterium (designated strain WJ7-1T) was isolated from activated sludge sampled in Suwon, Republic of Korea. 16S rRNA gene sequence analysis indicated that the isolate belongs to the genus Humibacter, family Microbacteriaceae, with a sequence similarity of 97.9?% to its nearest neighbour Humibacter albus KACC 20986T. Strain WJ7-1T contained menaquinone (MK)-10 (16.0?%), 11 (48.5?%) and 12 (35.5?%) as major respiratory quinones. The predominant cellular fatty acids (>15?%) were anteiso-C17?:?0, iso-C16?:?0 and anteiso-C15?:?0. The peptidoglycan of strain WJ7-1T contained the diagnostic diamino acid ornithine and 2,4-diaminobutyric acid alanine. Alanine, glutamic acid and glycine were also present in the cell wall. The characteristic whole-cell sugars present were glucose, galactose, xylose and rhamnose. The polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, an unidentified glycolipid and two unidentified phospholipids. Strain WJ7-1T possessed ginsenoside-converting (β-glucosidase) activity, which enabled it to transform ginsenoside protopanaxadiol-type Rb1 (one of the dominant active components of ginseng) to compound K. The genome size of strain WJ7-1T has 4.2 Mbp and the G+C content is 68.3?mol%. Average nucleotide identity, amino acid identity and digital DNA?DNA hybridization values between strain WJ7-1T and the closely related strain were 79.8, 36.1 and 23.5?%, respectively, indicating that strain WJ7-1T represents a novel species of the genus Humibacter. Strain WJ7-1T could be distinguished from the other members of the genus Humibacter by a number of chemotaxonomic and phenotypic characteristics. Based on polyphasic taxonomic analysis, strain WJ7-1T (=KACC 19729T=LMG 30802T) represents a novel species within the genus Humibacter, for which the name Humibacter ginsenosidimutans sp. nov. is proposed.
Keyword
Humibacter ginsenosidimutansPolyphasic taxonomy16S rRNA gene sequenceGenome sequenceBioconversion of ginsenoside
ISSN
1466-5026
Publisher
Microbiology Soc
DOI
http://dx.doi.org/10.1099/ijsem.0.005442
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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