Sexing of mouse embryos by chromosomal analysis = 염색체 분석에 의한 생쥐 수정란의 성감별

Cited 0 time in scopus
Metadata Downloads
Title
Sexing of mouse embryos by chromosomal analysis = 염색체 분석에 의한 생쥐 수정란의 성감별
Author(s)
Yong Mahn Han; J B Kim; H Y Park; K S Chung; Kyung Kwang Lee
Bibliographic Citation
Reproductive & Developmental Biology, vol. 10, no. 1, pp. 36-41
Publication Year
1986
Abstract
These experiments were carried out to obtain basic information necessary for sexing embryos by chromosomal analysis. To observe metaphase chromosomes, all embryos developed to blastocysts were cultured in Hoppe & Pitts’ medium containing 0.001% Colcemid under the gas phase of 5% CO! in air at 37 C for 2 hours. The sex chronjosome of mouse embryos shown normal development after culture in medium containing H-Y antiserum (10%,v/v) and complement (20%, v/v) also was confimed by chromosomal analysis. The results obtained in these experiments were summarized as follows: 1. Among 89 mouse blastocysts, the number of embryos identified to have XX and XY chromosome was 22(25%) and 25(28%), respectively and 42(47%) embryos were not identified. 2. Of total 40 mouse balstocysts cultured in medium containing H-Y antiserum and complement, 23(58%) embryos which were able to be discriminated their sex chromosomes were identified to be XX bearing embryos. 3. Sex chromosomes of a number of embryos subjected to chromosomal nalysis were not identified. This result may be due to absence or poor quality of metaphase spreads.
ISSN
I000-0006
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.