Production of interferon-gamma from human peripheral blood lymphocytes

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Production of interferon-gamma from human peripheral blood lymphocytes
Il Yup Chung; Young Il Yeom; Sang Ki Rhee; Tai Wha Chung; Moon Hi Han
Bibliographic Citation
Korean Biochemical Journal, vol. 19, no. 3, pp. 219-224
Publication Year
Interferon- 7 (IFN- 7) was produced from human peripheral blood lymphocytes (Hu-PBL) by stimulating them with various kinds of mitogens. T-cell mitogens such as Con A and PHA-M were much more potent than B-cell mitogen such as PWM in producing IFN- 7 from Hu-PBL. The combined treatment of TPA with these mitogens enhanced IFN- / production ten fold compared with the single treatment of each mitogen. Both DNA synthesis and IFN- 7 production yielded bell-shaped dose-response curves for a given population of Hu-PBL in response to Con A. The effect of fetal calf serum(FCS) on IFN- 7 production was found to be critical: titer of IFN- 7 produced was very low in the absence of FCS and reached peak point at 2% FCS, beginning to decrease precipitously with an increasing FCS concentration up to 20%. Using the relationship between IFN- 7 production and mitogenic stimulation, it was found that one of factors responsible for the decrease of IFN- 7 production at the high serum concentration could be the binding of Con A to serum proteins which rendered Con A to be removed from the production system.
Korea Soc-Assoc-Inst
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Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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